Results 51 to 60 of about 40,725 (187)
Heliyon, 2020 Binding interactions of boronic acid derivatives viz. 2-Methylphenylboronic acid (B1) and 3-Methoxyphenylboronic acid (B2) with mono saccharides (arabinose, fructose and galactose) and disaccharides (sucrose, lactose and maltose) in aqueous condition at ...
Raveendra Melavanki +4 more
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Model of maltose-binding protein/chemoreceptor complex supports intrasubunit signaling mechanism [PDF]
Proceedings of the National Academy of Sciences, 1999 The Tar protein of Escherichia coli is unique among known bacterial chemoreceptors in that it generates additive responses to two very disparate ligands, aspartate and maltose. Aspartate binds directly to the periplasmic (extracytoplasmic) domain of Tar. Maltose first binds to maltose-binding protein (MBP). MBP then
Y, Zhang +5 more
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Stimulation of the Maltose Transporter ATPase by Unliganded Maltose Binding Protein [PDF]
Biochemistry, 2009 ATP hydrolysis by the maltose transporter (MalFGK(2)) is regulated by maltose binding protein (MBP). Binding of maltose to MBP brings about a conformational change from open to closed that leads to a strong stimulation of the MalFGK(2) ATPase. In this study, we address the long-standing but enigmatic observation that unliganded MBP is also able to ...
Gould, Alister D. +2 more
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Several Affinity Tags Commonly Used in Chromatographic Purification
Journal of Analytical Methods in Chemistry, 2013 Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes ...
Xinyu Zhao, Guoshun Li, Shufang Liang
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STAR ProtocolsSummary: Polyphosphate exhibits a unique post-translational modification-like function, known as histidine polyphosphate modification (HPM), marked by a robust non-covalent interaction with histidine repeat proteins.
Kirsten Lehotsky +2 more
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Crystal structure of ORF210 from E. coli O157:H1 phage CBA120 (TSP1), a putative tailspike protein. [PDF]
PLoS ONE, 2014 Bacteriophage tailspike proteins act as primary receptors, often possessing endoglycosidase activity toward bacterial lipopolysaccharides or other exopolysaccharides, which enable phage absorption and subsequent DNA injection into the host. Phage CBA120,
Chen Chen +5 more
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Easy mammalian expression and crystallography of maltose-binding protein-fused human proteins
Journal of Structural Biology, 2016 We present a strategy to obtain milligrams of highly post-translationally modified eukaryotic proteins, transiently expressed in mammalian cells as rigid or cleavable fusions with a mammalianized version of bacterial maltose-binding protein (mMBP). This variant was engineered to combine mutations that enhance MBP solubility and affinity purification ...
Bokhove, Marcel +9 more
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Microbial Cell Factories, 2018 Background Bacterial expression and purification of recombinant proteins under homogeneous active form is often challenging. Fusion to highly soluble carrier proteins such as Maltose Binding Protein (MBP) often improves their folding and solubility, but ...
Anna Bonhoure +7 more
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Precursor maltose-binding protein is active in binding substrate.
Journal of Biological Chemistry, 1979 Precursor maltose-binding protein synthesized in vitro was shown to be active in binding maltose by affinity chromatography.
T, Ferenci, L L, Randall
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Revista Colombiana de Química, 2017 Recombinant proteins have become useful tools in biochemistry research. During their production, however, inclusion bodies (IB) appear, on the one hand, due to the high expression rate from the recombinant plasmids, which have high efficiency promoters ...
Carlos Alfonso Nieto Clavijo +2 more
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