Results 61 to 70 of about 936,446 (303)
Microbial Cell Factories, 2021 Background Proteins with novel functions or advanced activities developed by various protein engineering techniques must have sufficient solubility to retain their bioactivity.
Hyunjun Ko +7 more
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Location of tolerated insertions/deletions in the structure of the maltose binding protein [PDF]
FEBS Letters, 1993 In a previous study [(1987) J. Mol. Biol. 194, 663‐673], we isolated ten insertion/deletion mutants (indels) of the maltose binding protein for which the maltose binding constant was only a little or not at all affected. In this paper, we have localized these mutations in the recently solved three‐dimensional structure.
Maurice Hofnung +3 more
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Structural insight into the TRIAP1/PRELI-like domain family of mitochondrial phospholipid transfer complexes [PDF]
, 2015 The composition of the mitochondrial membrane is important for its architecture and proper function. Mitochondria depend on a tightly regulated supply of phospholipid via intra-mitochondrial synthesis and by direct import from the endoplasmic reticulum ...
Abid Ali, F +9 more
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BMC Biotechnology, 2020 Background Few bead-based multiplex assays have been described that detect antibodies against the protozoan parasite Toxoplasma gondii in large-scale seroepidemiological surveys. Moreover, each multiplex assay has specific variations or limitations, such
Sandra Klein, Daniel Stern, Frank Seeber
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Mutations in maltose-binding protein that alter affinity and solubility properties [PDF]
Applied Microbiology and Biotechnology, 2010 Maltose-binding protein (MBP) from Escherichia coli has been shown to be a good substrate for protein engineering leading to altered binding (Marvin and Hellinga, Proc Natl Acad Sci U S A 98:4955-4960, 2001a) and increased affinity (Marvin and Hellinga, Nat Struct Biol 8:795-798, 2001b; Telmer and Shilton, J Biol Chem 278:34555-34567, 2003). It is also
Pei-Chung Hsieh +2 more
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BioTechniques, 2012 We observed the presence of contaminating NADH oxidation activity in maltose binding protein (MBP) fusion proteins expressed in Escherichia coli and purified using conventional amylose resin-based affinity chromatography.
Fengguang Guo, Guan Zhu
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Evidence for a "wattle and daub" model of the cyst wall of entamoeba. [PDF]
PLoS Pathogens, 2009 The cyst wall of Entamoeba invadens (Ei), a model for the human pathogen Entamoeba histolytica, is composed of fibrils of chitin and three chitin-binding lectins called Jacob, Jessie3, and chitinase.
Anirban Chatterjee +6 more
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Engineering of Cyclodextrin Product Specificity and pH Optima of the Thermostable Cyclodextrin Glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1 [PDF]
, 1998 The product specificity and pH optimum of the thermostable cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes EM1 was engineered using a combination of x-ray crystallography and site-directed mutagenesis.
Bauke W. Dijkstra +44 more
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Scientific Reports, 2021 Cysteine-rich secretory proteins (CRISPs) are a subgroup of the CRISP, antigen 5 and PR-1 (CAP) superfamily that is characterized by the presence of a conserved CAP domain.
Jie Sheng +4 more
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Protein Delivery of an Artificial Transcription Factor Restores Widespread Ube3a Expression in an Angelman Syndrome Mouse Brain. [PDF]
, 2016 Angelman syndrome (AS) is a neurological genetic disorder caused by loss of expression of the maternal copy of UBE3A in the brain. Due to brain-specific genetic imprinting at this locus, the paternal UBE3A is silenced by a long antisense transcript ...
Adams, Alexa N +9 more
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