Results 61 to 70 of about 40,725 (187)

Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconventional use of oxidizing environments with a maltose binding protein-hexahistine dual tag to enhance membrane protein yield

Microbial Cell Factories, 2011
Background Obtaining membrane proteins in sufficient quantity for biophysical study and biotechnological applications has been a difficult task. Use of the maltose binding protein/hexahistidine dual tag system with E.coli as an expression host is ...
Straus Suzana K, Tait Andrew R
doaj   +1 more source

The Binding of Maltose to ‘Virgin’ Maltose‐Binding Protein is Biphasic [PDF]

European Journal of Biochemistry, 1975
The biphasic binding properties of the galactose‐binding and maltose‐binding proteins of Escherichia coli may be important in the functioning of these proteins as recognition components of chemoreceptors. However, Richarme and Kepes [Eur. J. Biochem. 45, 127‐133 (1974)] have suggested that the biphasic binding curve of the galactose‐binding protein may
openaire   +2 more sources

Defective secretion of maltose- and ribose-binding proteins caused by a truncated periplasmic protein in Escherichia coli [PDF]

, 1985
Regine Hengge, Winfried Boos
openalex   +1 more source

Enhancing butanol tolerance of Escherichia coli reveals hydrophobic interaction of multi-tasking chaperone SecB

Biotechnology for Biofuels, 2019
Background Escherichia coli has been proved to be one promising platform chassis for the production of various natural products, such as biofuels. Product toxicity is one of the main bottlenecks for achieving maximum production of biofuels.
Guochao Xu, Anning Wu, Lin Xiao, Ruizhi Han, Ye Ni   +4 more
doaj   +1 more source

Lactococcus lactis mutants resistant to lactococcin A and garvicin Q reveal missense mutations in the sugar transport domain of the mannose phosphotransferase system

Microbiology Spectrum
Lactococcin A is a bacteriocin from Lactococcus lactis that permeabilizes the membrane of sensitive lactococcal cells and requires the presence of the membrane-bound components IIC and IID of the mannose phosphotransferase system (man-PTS).
Marco J. van Belkum, Tamara Aleksandrzak-Piekarczyk, Tess Lamer, John C. Vederas   +3 more
doaj   +1 more source

Assessment of the Functional Regions of the Superantigen Staphylococcal Enterotoxin B

Toxins, 2013
The functional activity of superantigens is based on capacity of these microbial proteins to bind to both the β-chain of the T cell receptor (TcR) and the major histocompatibility complex (MHC) class II dimer.
Thomas J. Rogers, Lily Zhang
doaj   +1 more source

A large, general and modular DARPin–apoferritin scaffold enables the visualization of small proteins by cryo-EM

IUCrJ
Single-particle cryo-electron microscopy (cryo-EM) has emerged as an indispensable technique in structural biology that is pivotal for deciphering protein architectures. However, the medium-sized proteins (30–40 kDa) that are prevalent in both eukaryotic
Xin Lu, Ming Yan, Yang Cai, Xi Song, Huan Chen, Mengtan Du, Zhenyi Wang, Jia'an Li, Liwen Niu, Fuxing Zeng, Quan Hao, Hongmin Zhang   +11 more
doaj   +1 more source

A simple and sensitive detection of the binding ligands by using the receptor aggregation and NMR spectroscopy: a test case of the maltose binding protein [PDF]

, 2021
Young Kee Chae, Yoonjin Um, Hakbeom Kim
openalex   +1 more source

Active transport of maltose in membrane vesicles obtained from Escherichia coli cells producing tethered maltose-binding protein.

, 2020
D A Dean, J D Fikes, K Gehring, P J Bassford, H Nikaido   +4 more
openalex   +1 more source

Purification and Characterization of Maltose Binding Protein of Saccharomyces cerevisiae. [PDF]

, 1981
Maj-Rita Siro, Katriina Viitanen, Timo Lövgren, Herrick Baltscheffsky, Anders Rane   +4 more
openalex   +1 more source