Heterologous expression of mycobacterial Esx complexes in Escherichia coli for structural studies is facilitated by the use of maltose binding protein fusions. [PDF]
PLoS ONE, 2013 The expression of heteroligomeric protein complexes for structural studies often requires a special coexpression strategy. The reason is that the solubility and proper folding of each subunit of the complex requires physical association with other ...
Mark A Arbing +14 more
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Programming Xenon Diffusion in Maltose Binding Protein
SSRN Electronic Journal, 2022 Protein interiors contain void space that can bind small gas molecules. Determination of gas pathways and kinetics in proteins has been an intriguing and challenging task. Here, we combined computational methods and the hyperpolarized xenon-129 chemical exchange saturation transfer (hyper-CEST) NMR technique to investigate xenon (Xe) exchange kinetics ...
Zhuangyu Zhao +3 more
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Spectroscopic properties of an engineered maltose binding protein [PDF]
Protein Engineering Design and Selection, 1997 The maltose binding protein (MBP) has been site specifically labelled with a nitrobenzoxadiazole (NBD) group following mutation of a serine to a cysteine residue at position 337. The resulting protein shows a large ligand (maltose or beta-cyclodextrin) dependent increase in its steady-state fluorescence intensity.
Gianfranco Gilardi +4 more
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Accessing a hidden conformation of the maltose binding protein using accelerated molecular dynamics.
PLoS Computational Biology, 2011 Periplasmic binding proteins (PBPs) are a large family of molecular transporters that play a key role in nutrient uptake and chemotaxis in Gram-negative bacteria.
Denis Bucher +3 more
doaj +2 more sources
A useful epitope tag derived from maltose binding protein [PDF]
Protein Science, 2021 AbstractMaltose binding protein (MBP) is used in recombinant protein expression as an affinity and solubility tag. The monoclonal antibody B48 binds MBP tightly and has no cross‐reactivity to other proteins in an Escherichia coli lysate. This high level of specificity suggested that MBP contains an epitope that could prove useful as a purification and ...
Marine Lénon +6 more
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Further Studies on the Binding of Maltose to the Maltose‐Binding Protein of Escherichia coli [PDF]
European Journal of Biochemistry, 1976 Extensive binding studies, performed by equilibrium dialysis, suggest that the periplasmic maltosebinding protein of Escherichia coli is homogeneous with respect to its affinity towards maltose. This finding contradicts an earlier conclusion, which was based on insufficient data.
Maxime Schwartz +3 more
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Identification of a system required for the functional surface localization of sugar binding proteins with class III signal peptides in Sulfolobus solfataricus [PDF]
, 2007 The hyperthermophilic archaeon Sulfolobus solfataricus contains an unusual large number of sugar binding proteins that are synthesized as precursors with a class III signal peptide.
Albers S.V. +12 more
core +33 more sources
Both maltose-binding protein and ATP are required for nucleotide-binding domain closure in the intact maltose ABC transporter [PDF]
Proceedings of the National Academy of Sciences, 2008 The maltose transporter MalFGK 2 of Escherichia coli is a member of the ATP-binding cassette superfamily. A periplasmic maltose-binding protein (MBP) delivers maltose to MalFGK 2 and stimulates its ATPase activity.
Cédric Orelle +4 more
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Pharmaceutics, 2022 Protein modification with non-canonical amino acids (ncAAs) represents a useful technology to afford homogenous samples of bioconjugates with site-specific modification.
Alexander C. Williard +10 more
doaj +1 more source
Competitive interactions of ligands and macromolecular crowders with maltose binding protein. [PDF]
PLoS ONE, 2013 Cellular signaling involves a cascade of recognition events occurring in a complex environment with high concentrations of proteins, polysaccharides, and other macromolecules.
Andrew C Miklos +2 more
doaj +1 more source