Results 121 to 130 of about 73,040 (302)
Conformational selection in protein binding and function
, 2014 Protein binding and function often involves conformational changes. Advanced NMR experiments indicate that these conformational changes can occur in the absence of ligand molecules (or with bound ligands), and that the ligands may 'select' protein ...
Paul, Fabian, Weikl, Thomas R.
core +1 more source
Harnessing S. cerevisiae to advance the engineering of pentatricopeptide repeat proteins
The FEBS Journal, EarlyView.Heterologous expression systems have been instrumental in furthering our understanding of plant RNA editing proteins. In this commentary, we discuss how the establishment of yeast as a model for studying plant RNA editing by Ramanathan et al. could advance the engineering of pentatricopeptide repeat proteins, and how in return pentatricopeptide repeat ...
Farley M. Kwok van der Giezen, Ian Small
wiley +1 more source
Engineered Derivatives of Maltose-Binding Protein
, 2012 Maltose-binding protein (MBP), a member of the periplasmic binding protein family of Gram negative bacteria, is a versatile substrate for protein engineering. In common with other periplasmic proteins, it is extremely protease resistant, and it can fold properly in both the cytoplasmic and periplasmic compartments.
openaire +3 more sources
A Thioredoxin Domain-Containing Protein Interacts with Pepino mosaic virus Triple Gene Block Protein 1 [PDF]
, 2018 Pepino mosaic virus (PepMV) is a mechanically-transmitted tomato pathogen of importance worldwide. Interactions between the PepMV coat protein and triple gene block protein (TGBp1) with the host heat shock cognate protein 70 and catalase 1 (CAT1 ...
Khechmar, Souheyla +8 more
core +2 more sources
The FEBS Journal, EarlyView.Small protein MtrR is a regulator of the Mtr methyltransferase complex in Methanosarcina mazei. It binds specifically to the MtrA subunit and modulates Mtr activity in response to hydrogen (H2) availability. Deleting mtrR impairs growth in the presence but not absence of H2, indicating its role in directing methyl transfer toward an energy‐conserving ...
Tim Habenicht +6 more
wiley +1 more source
Journal of Integrative Plant Biology, EarlyView.During strawberry ripening, abscisic acid signaling activates SnRK2.6, which phosphorylates and degrades the JAZ12 repressor. This releases the MYC2 transcription factor, directly activating jasmonic acid biosynthesis genes. The resulting jasmonic acid accumulation drives aroma production, thereby coupling ripening with scent formation through this ...
Kenan Jia +10 more
wiley +1 more source
Soluble Cytoplasmic Expression and Purification of Immunotoxin HER2(scFv)-PE24B as a Maltose Binding Protein Fusion. [PDF]
Int J Mol Sci, 2021 Park S +7 more
europepmc +1 more source
Journal of Integrative Plant Biology, EarlyView.Auxin signaling regulates the formation of vegetative propagation corms in Gastrodia elata by regulating sugar‐acid interconversion, which in turn regulates polysaccharide and starch biosynthesis as well as biosynthesis of the bioactive metabolite gastrodin.
Qun Liu +9 more
wiley +1 more source
Journal of Molecular Biology, 2000 Recombinant maltose-binding protein from Thermotoga maritima (TmMBP) was expressed in Escherichia coli and purified to homogeneity, applying heat incubation of the crude extract at 75 degrees C. As taken from the spectral, physicochemical and binding properties, the recombinant protein is indistinguishable from the natural protein isolated from the ...
Wassenberg, D. +2 more
openaire +3 more sources
A simple and sensitive detection of the binding ligands by using the receptor aggregation and NMR spectroscopy: a test case of the maltose binding protein. [PDF]
J Biomol NMR, 2021 Chae YK, Um Y, Kim H.
europepmc +1 more source