Purity of maltose-binding protein – Recombinant streptavidin expressed in Escherichia coli BL21 (pD861-MBP: 327892) [PDF]
Journal of Advanced Pharmaceutical Technology & Research, 2022 Nearly 95% of streptavidin which is expressed in Escherichia coli found as an inclusion body. Protein expressed in an inclusion body form requires further steps for the folding process related to its purification.
Toto Subroto +5 more
doaj +2 more sources
Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems. [PDF]
PLoS ONE, 2016 Recombinant proteins are commonly expressed in eukaryotic expression systems to ensure the formation of disulfide bridges and proper glycosylation. Although many proteins can be expressed easily, some proteins, sub-domains, and mutant protein versions ...
Raphael Reuten +7 more
doaj +2 more sources
In Silico Generation of Peptides by Replica Exchange Monte Carlo: Docking-Based Optimization of Maltose-Binding-Protein Ligands. [PDF]
PLoS ONE, 2015 Short peptides can be designed in silico and synthesized through automated techniques, making them advantageous and versatile protein binders. A number of docking-based algorithms allow for a computational screening of peptides as binders.
Anna Russo +8 more
doaj +2 more sources
Molecular analysis of cyclic α-maltosyl-(1→6)-maltose binding protein in the bacterial metabolic pathway. [PDF]
PLoS ONE, 2020 Cyclic α-maltosyl-(1→6)-maltose (CMM) is a cyclic glucotetrasaccharide with alternating α-1,4 and α-1,6 linkages. Here, we report functional and structural analyses on CMM-binding protein (CMMBP), which is a substrate-binding protein (SBP) of an ABC ...
Masaki Kohno +6 more
doaj +2 more sources
Prokaryotic Soluble Overexpression and Purification of Human VEGF165 by Fusion to a Maltose Binding Protein Tag. [PDF]
PLoS ONE, 2016 Human vascular endothelial growth factor (VEGF) is a key regulator of angiogenesis and plays a central role in the process of tumor growth and metastatic dissemination. Escherichia coli is one of the most common expression systems used for the production
Minh Tan Nguyen +13 more
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Correction: Prokaryotic soluble overexpression and purification of human VEGF165 by fusion to a maltose binding protein tag. [PDF]
PLoS ONE, 2017 [This corrects the article DOI: 10.1371/journal.pone.0156296.].
Minh Tan Nguyen +13 more
doaj +2 more sources
A Maltose-Binding Protein Fusion Construct Yields a Robust Crystallography Platform for MCL1. [PDF]
PLoS ONE, 2015 Crystallization of a maltose-binding protein MCL1 fusion has yielded a robust crystallography platform that generated the first apo MCL1 crystal structure, as well as five ligand-bound structures.
Matthew C Clifton +17 more
doaj +2 more sources
Maltose-binding protein fusion allows for high level bacterial expression and purification of bioactive mammalian cytokine derivatives. [PDF]
PLoS ONE, 2014 Fusokines are chimeric proteins generated by the physical coupling of cytokines in a single polypeptide, resulting in proteins with highly pleiotropic activity and the potential to treat cancer and autoimmune ailments.
Andrea Pennati +2 more
doaj +2 more sources
Conformational Dynamics in the Binding-Protein-Independent Mutant of the Escherichia coli Maltose Transporter, MalG511, and Its Interaction with Maltose Binding Protein [PDF]
Biochemistry, 2018 Ruchika Bajaj +3 more
openalex +3 more sources
Stabilization versus flexibility: Detergent-dependent trade-offs in neurotensin receptor 1 GPCR ensembles. [PDF]
Protein SciAbstract Detergents provide essential membrane‐mimetic environments for studying G protein‐coupled receptors (GPCRs), but their molecular impact on receptor energetics remains incompletely understood. We combined ligand binding, thermostability measurements, and atomistic molecular dynamics to dissect detergent‐ versus ligand‐driven stabilization in a ...
Bower JB +6 more
europepmc +2 more sources