Results 271 to 280 of about 73,040 (302)

Production of the chimeric-binding protein, maltose-binding protein-protein a, by gene fusion

Journal of Biotechnology, 1995
A fusion protein between maltose-binding protein (MBP) and staphylococcal protein A (SpA) was genetically produced. The gene fusion plasmid, pMALPA2, was constructed by inserting the protein A gene into an expression vector of maltose-binding protein in frame, and was expressed efficiently in Escherichia coli.
E, Kobatake, Y, Ikariyama, M, Aizawa
openaire   +2 more sources

Periplasmic binding protein based biosensors 1. Preliminary study of maltose binding protein as sensing element for maltose biosensor

Biosensors and Bioelectronics, 1991
Abstract The maltose binding protein has been isolated from Escherichia coli and immobilized onto pore-controlled glass using three different chemical methods. All three methods immobilized similar quantities of protein but with different specific activities.
Li Qing Zhou, Anthony E.G. Cass
openaire   +1 more source

Membrane Protein Topology Determination by Proteolysis of Maltose Binding Protein Fusions

Analytical Biochemistry, 1993
A method is presented for determining the topology of Escherichia coli inner membrane proteins that is based on proteolysis of fusion proteins between maltose binding protein (MBP) and the membrane protein of interest. Fusion proteins are constructed wherein the MBP domain is fused upstream of the membrane protein domain.
K W, Miller, P L, Konen, J, Olson, K M, Ratanavanich   +3 more
openaire   +2 more sources

Induced fit of passenger proteins fused to Archaea maltose binding proteins

Biochemical and Biophysical Research Communications, 2006
Maltose binding proteins (MBPs) are used as carriers for improving the solubility of passenger proteins. Our results indicate that the higher solubility of the fusions correlates with their elevated heat stability. Fusions of the otherwise thermo-sensitive GFP with MBPs from Archaea, but not GST-GFP and Escherichia coli MBP-GFP, maintained their ...
He, Huang, Jing, Liu, Ario, de Marco
openaire   +2 more sources

Export of the periplasmic maltose-binding protein ofEscherichia coli

Journal of Bioenergetics and Biomembranes, 1990
The export of the maltose-binding protein (MBP), the malE gene product, to the periplasm of Escherichia coli cells has been extensively investigated. The isolation of strains synthesizing MalE-LacZ hybrid proteins led to a novel genetic selection for mutants that accumulate export-defective precursor MBP (preMBP) in the cytoplasm.
openaire   +2 more sources

The contemporary management of cancers of the sinonasal tract in adults

Ca-A Cancer Journal for Clinicians, 2023
Rajat Thawani
exaly  

Highly accurate protein structure prediction with AlphaFold

Nature, 2021
John M Jumper, Richard Evans, Alexander Pritzel   +2 more
exaly  

Maltose-Binding Protein as a Solubility Enhancer

2003
Jeffrey D, Fox, David S, Waugh
openaire   +2 more sources

Accurate prediction of protein structures and interactions using a three-track neural network

Science, 2021
Minkyung Baek, Frank DiMaio, Ivan Anishchenko   +2 more
exaly  

Highly accurate protein structure prediction for the human proteome

Nature, 2021
Kathryn Tunyasuvunakool, Jonas Adler, Zachary Wu   +2 more
exaly