Thermodynamics of maltose binding protein unfolding [PDF]
Protein Science, 1997 AbstractThe maltose binding protein (MBP or MalE) of Escherichia coli is the periplasmic component of the transport system for malto‐oligosaccharides. It is used widely as a carrier protein for the production of recombinant fusion proteins. The melting of recombinant MBP was studied by differential scanning and titration calorimetry and fluorescence ...
V, Novokhatny, K, Ingham
openaire +2 more sources
α-Glucosidase Inhibitory Potential of <i>Citrus reticulata</i> Peel-Derived Flavonoids-A Prelude for the Management of Type 2 Diabetes. [PDF]
Food Sci NutrFlavonoids derived from Citrus reticulate peel inhibit α‐glucosidase activity, delaying carbohydrate digestion and reducing glucose absorption, thereby indicating their potential as functional food components for type 2 diabetes management. ABSTRACT α‐Glucosidase inhibitors (AGIs) are compounds used to treat type 2 diabetes (T2D) by preventing the ...
Baloyi IT, Rabbad AH, Gama NH, Malgas S.
europepmc +2 more sources
Discovery of an auto-regulation mechanism for the maltose ABC transporter MalFGK2. [PDF]
PLoS ONE, 2012 The maltose transporter MalFGK(2), together with the substrate-binding protein MalE, is one of the best-characterized ABC transporters. In the conventional model, MalE captures maltose in the periplasm and delivers the sugar to the transporter.
Huan Bao, Franck Duong
doaj +1 more source
In vitro recombination of non-homologous genes can result in gene fusions that confer a switching phenotype to cells. [PDF]
PLoS ONE, 2011 Regulation of protein activity is central to the complexity of life. The ability to regulate protein activity through exogenously added molecules has biotechnological/biomedical applications and offers tools for basic science.
Richard A Heins +3 more
doaj +1 more source
Computational analysis of maltose binding protein translocation [PDF]
Philosophical Magazine, 2011 We propose a computational model for the study of maltose binding protein translocation across alpha-hemolysin nanopores. The phenomenological approach simplifies both the pore and the polypeptide chain; however it retains the basic structural protein-like properties of the maltose binding protein by promoting the correct formation of its native key ...
CHINAPPI, MAURO +2 more
openaire +3 more sources
Stimulation of the Maltose Transporter ATPase by Unliganded Maltose Binding Protein [PDF]
Biochemistry, 2009 ATP hydrolysis by the maltose transporter (MalFGK(2)) is regulated by maltose binding protein (MBP). Binding of maltose to MBP brings about a conformational change from open to closed that leads to a strong stimulation of the MalFGK(2) ATPase. In this study, we address the long-standing but enigmatic observation that unliganded MBP is also able to ...
Gould, Alister D. +2 more
openaire +4 more sources
Structure of an engineered β-lactamase maltose binding protein fusion protein: insights into heterotropic allosteric regulation. [PDF]
PLoS ONE, 2012 Engineering novel allostery into existing proteins is a challenging endeavor to obtain novel sensors, therapeutic proteins, or modulate metabolic and cellular processes.
Wei Ke +8 more
doaj +1 more source
UV difference spectroscopy of ligand binding to maltose‐binding protein [PDF]
FEBS Letters, 1992 We have used UV absorbance spectroscopy to study the binding of linear and circular maltodextrins to maltose‐binding protein (MBP). Titrations with maltose yield three ??? points in the difference spectrum of MBP, consistent with two protein conformations: ligand‐free and ligand‐bound.
Gehring, Kalle +2 more
openaire +2 more sources
Backbone assignment of crystalline E. coli maltose binding protein [PDF]
Biomolecular NMR Assignments, 2021 The E.coli maltose binding protein (MBP) is a 42.5 kDa molecule widely employed in many biotechnology applications. Because of its molecular size, it has become the main model system for the development of solution NMR methods adapted to large biomolecular targets.
Schubeis, Tobias +2 more
openaire +2 more sources
Macrophage‐derived MLKL in alcohol‐associated liver disease: Regulation of phagocytosis
Hepatology, EarlyView., 2022 EtOH causes leaky gut allowing bacteria and PAMPs into the liver, resulting in hepatic inflammation and injury. We demonstrate that LPS induces STAT1‐mediated expression and phosphorylation of MLKL in macrophages and identify a novel function that myeloid MLKL translocates to phagosomes and lysosomes and regulates phagocytosis, which contributes to the
Xiaoqin Wu +16 more
wiley +1 more source