Results 71 to 80 of about 73,040 (302)

Controlled Delivery and Light‐Induced Release of Magic Spot Nucleotides in Escherichia coli

Angewandte Chemie International Edition, EarlyView.
ABSTRACT The “magic spot” nucleotides (MSNs) ppGpp and pppGpp constitute bacterial alarmones that orchestrate the conserved stringent response, a global regulatory mechanism enabling bacteria to adapt to nutrient deprivation and other environmental stresses. Current strategies to manipulate MSN levels rely mainly on genetic or environmental approaches,
Christoph Popp, Patrick Moser, Anselm Schwoerbel, Xinwei Liu, Johannes Freitag, Pinku Sarmah, Isabel Prucker, Robert Zscherp, Xuan Wang, Philipp Klahn, Hans‐Georg Koch, Gert Bange, Henning J. Jessen   +12 more
wiley   +1 more source

Solution NMR studies of periplasmic binding proteins and their interaction partners

Biomolecular Concepts, 2011
Periplasmic binding proteins (PBPs) are a crucial part of ATP-binding cassette import systems in Gram-negative bacteria. Central to their function is the ability to undergo a large-scale conformational rearrangement from open-unliganded to closed ...
Pistolesi Sara, Tjandra Nico, Bermejo Guillermo A.   +2 more
doaj   +1 more source

Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process

Microbial Cell Factories, 2019
Background The production of therapeutically active single chain variable fragment (scFv) antibody is still challenging in E. coli due to the aggregation propensity of recombinant protein into inclusion bodies (IBs).
Animesh Sarker, Abhishek Singh Rathore, Rinkoo Devi Gupta   +2 more
doaj   +1 more source

Crystal structure of human NLRP12 PYD domain and implication in homotypic interaction. [PDF]

PLoS ONE, 2018
NLRP12 is a NOD-like receptor that plays multiple roles in both inflammation and tumorigenesis. Despite the importance, little is known about its mechanism of action at the molecular level.
Tengchuan Jin, Mo Huang, Jiansheng Jiang, Patrick Smith, Tsan Sam Xiao   +4 more
doaj   +1 more source

A point mutation of integrin beta 1 subunit blocks binding of alpha 5 beta 1 to fibronectin and invasin but not recruitment to adhesion plaques. [PDF]

, 1992
A point mutation in a highly conserved region of the beta 1 subunit, Asp130 to Ala (D130A) substitution, abrogates the Arg-Gly-Asp (RGD)-dependent binding of alpha 5 beta 1 to fibronectin (FN) without disrupting gross structure or heterodimer assembly ...
Ginsberg, MH, Mandelman, D, Puzon, W, Takada, Y, Ylänne, J   +4 more
core   +2 more sources

Exploring the Roles of Tuna‐Associated Acinetobacter sp. YFT067: Implications for Host Health and Metabolism

Animal Research and One Health, EarlyView.
This study firstly isolated a tuna‐derived gut probiotic Acinetobacter seifertii YFT067. Dietary administration of YFT067 played significant roles in promoting growth performance, SCFAs production, lipid absorption, and metabolism of the host, indicating YFT067 as a promising probiotic candidate for enhancing tuna aquaculture productivity through ...
Ying Zou, Hai Huang, Qiongyao Fu, Zhiyuan Lu, Zhiqiang Guo   +4 more
wiley   +1 more source

Computer‐Aided Functional Oligosaccharide Screening and the Regulatory Role in Lipid Metabolism

Food Bioengineering, EarlyView.
ABSTRACT This study investigated the lipid‐lowering effects and intervention mechanisms of structurally diverse functional oligosaccharides on non‐alcoholic fatty liver disease (NAFLD). By integrating computational tools such as molecular docking and molecular dynamics simulations (MDS), a comprehensive “in silico‐in vitro‐in vivo” tri‐dimensional ...
Rui Jing, Shanshan Deng, Mengyao Zhao, Liming Zhao   +3 more
wiley   +1 more source

MILKSHAKE: novel validation method for antibodies to post-translationally modified targets by surrogate Western blot

BioTechniques, 2022
Antibody (Ab) validation is the procedure in which an Ab is thoroughly assayed for sensitivity and specificity in a given application. Validation of Abs against post-translationally modified (PTM) targets is particularly challenging because it requires ...
Kezzia S Jones, Amanda E Chapman, Holland A Driscoll, Emily P Fuller, Meghan Kelly, Xiaofeng Li, Sourour Mansour, Shannon L McBride, Qi Zhao, Michael Weiner, Mary R Ferguson   +10 more
doaj   +1 more source

Periplasmic maltose-binding protein confers specificity on the outer membrane maltose pore of Escherichia coli [PDF]

Journal of Bacteriology, 1980
ompB mutants of Escherichia coli K-12 are markedly deficient in porin in their outer membrane. This results in a decreased rate of uptake for many substrates: the maltose pore (lambda receptor) can in some circumstances, in the absence of the periplasmic maltose-binding protein, compensate for the consequent defects in permeability to lactose, mannitol,
M W, Heuzenroeder, P, Reeves
openaire   +2 more sources

Antioxidant Activity and Inhibition of Carbohydrate Hydrolyzing Enzymes by Underutilized Parts From Some Plant‐Based Foods and Their Effect on Postprandial Hyperglycemia

Food Chemistry International, EarlyView.
The underutilized parts viz: pawpaw seed, banana, plantain, and potato peels showed promising antioxidant and inhibition of α‐amylase and α‐glucosidase activities (IC50 values ≤ 100 μg/mL). Similarly, pawpaw seed, banana, plantain, and potato peels reduced postprandial hyperglycemia in sucrose‐ and maltose‐induced hyperglycemia in rats.
Aminu Mohammed, Buhari Shehu Saidu, Nuhu Aliyu, Michael Peter Simon   +3 more
wiley   +1 more source