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Extraction and Deproteinization of Mannan Oligosaccharides

open access: yesZeitschrift Fur Naturforschung - Section C Journal of Biosciences, 2010
Extraction of mannan oligosaccharides from the yeast cell wall and methods of deproteinization were studied. We extracted crude mannan oligosaccharides by the dilute alkali- Sevage method. The percentages of deproteinization and mannan oligosaccharide loss were compared as indexes using the Sevage method, trichloroacetic acid method, and hydrochloric ...
Gang L Huang
exaly   +3 more sources
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Biosynthesis of yeast mannan. Characterization of mannan-synthesizing enzyme systems from mutants defective in mannan structure

Folia Microbiologica, 1976
The yeast Saccharomyces cerevisiae X2180-1A (wild) and its mutants X2180-1A-4 (mnn 1) and X2180-1A-5 (mnn 2) defective in mannan biosynthesis were used as enzyme sources to catalyze in vitro mannosyl transfer from GDP-[14C-U]-mannose to endogenous glycoproteins as well as to exogenous, low-molecular weight acceptors.
V, Farkas, S, Bauer
openaire   +2 more sources

Immunostimulatory properties of coffee mannans

Molecular Nutrition & Food Research, 2009
AbstractCoffee infusion mannans are acetylated polysaccharides containing single Galp and Araf residues as side chains of a β‐(1 → 4)‐Manp backbone. These mannans are structurally similar to the bioactive acetylated mannans from Aloe vera (AV). In this study, acetylated mannans were obtained from two coffee infusions prepared from light and dark ...
Joana, Simões   +5 more
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A MANNAN PRODUCED BY BACILLUS POLYMYXA

Canadian Journal of Chemistry, 1959
During the fermentation of glucose, sucrose, or lactose by Bacilluspolymyxa, a mixture of polysaccharides is formed, one of which has been isolated by mild procedures and shown to contain mannose as the only sugar together with approximately 13% protein. Acid hydrolysis of the methylated mannan gave 2,3,4,6-tetra-O-methyl-D-mannose, 2,4,6-tri-O-methyl-
Ball, D.H., Adams, G.A.
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Mannosylphosphate transfer to yeast mannan

Biochimica et Biophysica Acta (BBA) - General Subjects, 1999
Mannoproteins located in the outermost layer of yeast cell wall determine the wall's porosity and thereby regulate leakage of proteins from the periplasmic space and entrance of macromolecules from the environment. In several yeasts, including Saccharomyces cerevisiae, the glycan portion of mannoproteins is composed not only of neutral oligosaccharides
Y, Jigami, T, Odani
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A MANNAN PRODUCED BY BACILLUS POLYMYXA

Canadian Journal of Biochemistry and Physiology, 1956
Null
G A, ADAMS, C T, BISHOP, D, MURPHY
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The enzymic synthesis of yeast mannan

Biochemical and Biophysical Research Communications, 1963
Abstract When guanosine diphosphate mannose (GDP-mannose) was first isolated from yeast, it was proposed that this compound might be the precursor of mannan ( Cabib and Leloir, 1954 ). Evidence supporting this hypothesis will be outlined in the present communication.
I D, ALGRANATI, H, CARMINATTI, E, CABIB
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An approach to the sequencing of yeast mannan

Biochemical and Biophysical Research Communications, 1975
Summary A “nearest neighbor” analysis of the products of partial acetolysis of yeast mannan was carried out in an attempt to learn whether the side chains in the polysaccharide are ordered in a sequence or occur randomly. The results suggest that the arrangement is nonrandom, but they do not give unequivocal support to a single repeating sequence.
L, Rosenfeld, C E, Ballou
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A procoagulant chemically sulfated mannan

Carbohydrate Polymers, 2016
Disorders of hemostasis can produce innumerous problems. Polysaccharides have been studied both as anticoagulant and as procoagulant agents. A mannan with a main chain of α-(1 → 6)-linked-Manp units, branched at O-2 mainly by side-chains of 2-O-linked-α-Manp units was chemically sulfated, structurally characterized by NMR and GC-MS (methylation ...
Ana Helena P, Gracher   +3 more
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