Results 81 to 90 of about 2,720 (255)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

The first 50 textbooks of Aesthetics in Spain

open access: yesHistoria de la Educación, 2013
The article presents the manuals that introduced the study of aesthe - tics in Spanish classrooms. These books are designed for the student. Generally were class notes, corrected and augmented.
Ignasi ROVIRÓ ALEMANY
doaj  

A MANUAL OF BACTERIOLOGY. [PDF]

open access: yesJournal of the American Chemical Society, 1907
n ...
openaire   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Proposal for a medical emergency, diagnosis and treatment manual for intern doctors

open access: yesActa Médica del Centro, 2010
A descriptive cross-sectional study, with a qualitative approach, was carried out with the aim of proposing a manual of medical emergencies, diagnosis and treatment, used for consultation and easy to handle.
Daniel Sánchez Currás   +5 more
doaj  

YIPFα1A expression is regulated by multilayered molecular mechanisms

open access: yesFEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji   +2 more
wiley   +1 more source

Un manual de urbanidad para los hispanoamericanos

open access: yesKipus: Revista Andina de Letras y Estudios Culturales, 2012
Uno de los libros más leídos por los hispanoamericanos desde mediados del siglo XIX, es el Manuel de urbanidad, de Manuel Antonio Carreño. Hasta hace pocas décadas, fue texto de lectura obligatoria en la mayoría de las escuelas hispanohablantes de ...
Mirla Alcibíades
doaj  

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

open access: yesFEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner   +7 more
wiley   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

open access: yesFEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young   +7 more
wiley   +1 more source

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