Results 91 to 100 of about 257,611 (263)

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

An Adaptive Whole‐Body Control Approach for Dynamic Obstacle Avoidance of Mobile Manipulators for Human‐Centric Smart Manufacturing

open access: yesIET Collaborative Intelligent Manufacturing
In human‐centric smart manufacturing (HCSM), the robot's dynamic obstacle avoidance function is crucial to ensuring human safety. Unlike the static obstacle avoidance of manipulators or mobile robots, the dynamic obstacle avoidance in mobile manipulators
Yong Tao   +6 more
doaj   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

open access: yesFEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão   +3 more
wiley   +1 more source

Production‐Logistics Synchronisation Mechanism and Method of Cellular Assembly Systems Based on Digital‐Twin and Out‐of‐Order Execution

open access: yesIET Collaborative Intelligent Manufacturing
In the realm of customised production modes, where dynamic disturbances are frequent, synchronised operations between production and logistics in cellular assembly systems play a pivotal role in swiftly responding to rapidly evolving personalised demands.
Weijie Zeng   +4 more
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

open access: yesFEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard   +12 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

open access: yesFEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya   +2 more
wiley   +1 more source

Identification of Critical Machine in Textile Industry Using Reliability‐Centred Maintenance and Failure Mode and Effects Analysis—A Case Study of Bahir Dar Textile's Share Company

open access: yesThe Journal of Engineering
Reliability‐Centred Maintenance (RCM) is an economical maintenance approach that places more emphasis on sustaining system functionality than just fixing individual hardware parts.
Befekadu Zeleke Asfaw   +3 more
doaj   +1 more source

Security Framework for Network-Based Manufacturing Systems with Personalized Customization: An Industry 4.0 Approach. [PDF]

open access: yesSensors (Basel), 2023
Hammad M   +7 more
europepmc   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

open access: yesFEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

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