Results 111 to 120 of about 2,270,482 (341)

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

open access: yesFEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

The pseudo-Goldstone mass spectrum

open access: yesPhysics Letters B, 1992
In schemes of strong electroweak symmetry breaking, independently of the details of the underlying fundamental theory, we develop a framework for the quantitative evaluation of the pseudo-Goldstone masses, including, beside the gauge contributions, also the contributions from those interactions which are responsible for the masses of the ordinary ...
Raoul Gatto   +6 more
openaire   +3 more sources

The E3 ubiquitin ligase, RNF219, suppresses CNOT6L expression to exhibit antiproliferative activity

open access: yesFEBS Open Bio, EarlyView.
We identified RNF219 as a CCR4‐NOT complex‐interacting E3 ubiquitin ligase that targets the CCR4‐NOT subunit CNOT6L for ubiquitination. RNF219 directly binds to the DUF3819 domain of CNOT1 through its putative α‐helix spanning amino acids 521–542. Our findings also suggest that antiproliferative activity of RNF219 is at least partially mediated by ...
Shou Soeda   +9 more
wiley   +1 more source

A mass spectrum-oriented computational method for ion mobility-resolved untargeted metabolomics. [PDF]

open access: yesNat Commun, 2023
Luo M   +6 more
europepmc   +1 more source

On optical imaging through aircraft turbulent boundary layers [PDF]

open access: yes, 1980
Optical resolution quality as affected by aircraft turbulent boundary layers is analyzed. Wind-tunnel data was analyzed to obtained the variation of boundary layer turbulence scale length and mass density rms fluctuations with Mach number.
Sutton, G. W.
core   +1 more source

Probing Symmetry-Breaking Pattern Using Sfermion Masses

open access: yes, 1993
We study the mass spectrum of superparticles within supersymmetric grand unified models. For gaugino masses, it is pointed out that the GUT-relation in the $SU(5)$ model is applicable to a more general case where a grand-unified gauge group breaks down ...
Abazov   +38 more
core   +2 more sources

Structural and functional characterization of chitinase from carnivorous plant Drosera adelae

open access: yesFEBS Open Bio, EarlyView.
A class I chitinase from the carnivorous plant Drosera adelae was expressed and purified using a yeast system, revealing high enzymatic activity. Structural analyses of the catalytic and chitin‐binding domains identified key tyrosine residues involved in substrate binding, offering insights into the enzyme's adaptation for insect digestion.
Kazunari Yoneda   +7 more
wiley   +1 more source

Mass function of haloes: scale invariant models [PDF]

open access: yes, 2009
Press-Schechter theory gives a simple, approximate functional form of the mass function of dark matter haloes. Sheth and Tormen (ST) refined this mass function to give an improved analytical fit to results of N-body simulations.
Bagla, J. S.   +2 more
core   +1 more source

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