Results 61 to 70 of about 194,577 (309)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Mathematical Programming for Data Mining: Formulations and Challenges [PDF]

, 1998
This paper is intended to serve as an overview of a rapidly emerging research and applications area. In addition to providing a general overview, motivating the importance of data mining problems within the area of knowledge discovery in databases, our
Fayyad, Usama M., Fayyad, Usama, O. L. Mangasarian, Mangasarian, Olvi, Usama M. Fayyad, P. S. Bradley, Mangasarian, O.L., Bradley, P.S.   +7 more
core   +1 more source

Optimal Selection of Conductors in Three-Phase Distribution Networks Using a Discrete Version of the Vortex Search Algorithm

Computation, 2021
In this study, a new methodology is proposed to perform optimal selection of conductors in three-phase distribution networks through a discrete version of the metaheuristic method of vortex search.
John Fernando Martínez-Gil, Nicolas Alejandro Moyano-García, Oscar Danilo Montoya, Jorge Alexander Alarcon-Villamil   +3 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Simulation-based solution of stochastic mathematical programs with complementarity constraints: Sample-path analysis [PDF]

We consider a class of stochastic mathematical programs with complementarity constraints, in which both the objective and the constraints involve limit functions or expectations that need to be estimated or approximated.
Birbil, S.I., Gürkan, G., ListeÅŸ, O.   +2 more
core   +4 more sources

Intelligent Tutoring Systems for Adult Learning in STEM Disciplines

New Directions for Adult and Continuing Education, EarlyView.
ABSTRACT Intelligent tutoring systems (ITS) are reshaping adult learning in STEM by providing adaptive, data‐driven instruction across classrooms, workplaces, and informal environments. In the context of ITS, this article compares generative AI, which creates personalized explanations and practice materials, with explainable AI, which focuses on ...
Jill Zarestky, Amanda R. Lager Gleason
wiley   +1 more source

Multidisciplinary design optimization framework for the pre design stage [PDF]

, 2010
Presented is a novel framework for performing flexible computational design studies at preliminary design stage. It incorporates a workflow management device (WMD) and a number of advanced numerical treatments, including multi-objective optimization ...
Balachandran, Libish Kalathil, Padulo, Mattia, Paolo Fantini, Nunez, Marco, Marin Guenov, Libish Balachandran, Guenov, Marin D., Maginot, Jeremy, Marco Nunez, Mattia Padulo, Jeremy Maginot, Fantini, Paolo   +11 more
core   +1 more source

19th International Conference on Mathematical Optimization Theory and Operations Research [PDF]

, 2020
This book constitutes the proceedings of the 19th International Conference on Mathematical Optimization Theory and Operations Research, MOTOR 2020, held in Novosibirsk, Russia, in July 2020.
Kalyagin, Valery, Khachay, Michael, Kononov, Alexander, Pardalos, Panos   +3 more
core   +1 more source

The SCIP Optimization Suite 8.0.0 [PDF]

, 2022
The SCIP Optimization Suite provides a collection of software packages for mathematical optimization centered around the constraint integer programming framework ...
Maher, Stephen J., Wellner, Philipp, Gamrath, Gerald, Wegscheider, Fabian, Bestuzheva, Ksenia, Lübbecke, Marco, Pfetsch, Marc E., Eifler, Leon, University of Exeter, Halbig, Katrin, Müller, Benjamin, Technische Universität Darmstadt, University of Twente, Gottwald, Leona, van Doornmalen, Jasper, Eindhoven University of Technology, Hendel, Gregor, Rehfeldt, Daniel, Schlein, Steffan, Combinatorial Optimization, Besançon, Mathieu, Sofranac, Boro, Gaul, Oliver, Gleixner, Ambros, Schlösser, Franziska, Serrano, Felipe, Witzig, Jakob, Zuse Institute Berlin, GAMS Software GmbH, van Doornmalen, Jasper; id_orcid, Vigerske, Stefan, Hoen, Alexander, Hojny, Christopher, Chen, Wei-Kun, Turner, Mark, Beijing Institute of Technology, Graczyk, Christoph, Shinano, Yuji, Weninger, Dieter, Donkiewicz, Tim, van der Hulst, Rolf, Matter, Frederic, Chimela, Antonia, Mühmer, Erik, RWTH Aachen University, Koch, Thorsten, University of Erlangen-Nuremberg   +46 more
core   +1 more source