Results 41 to 50 of about 10,220,677 (246)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

SUBSTITUTIONS, TILING DYNAMICAL SYSTEMS AND MINIMAL SELF-JOININGS

, 2014
We investigate substitution subshifts and tiling dynamical systems arising from the substitutions (1) theta : 0 -> 001, 1 -> 11001 and (2) eta : 0 > 001, 1 -> 11100. We show that the substitution subshifts arising from 61 and n have minimal self-joinings
Son, Younghwan
core   +1 more source

Pod systems : an equivariant ordinary differential equation approach to dynamical systems on a spatial domain [PDF]

, 1996
We present a class of systems of ordinary differential equations (ODEs), which we call 'pod systems', that offers a new perspective on dynamical systems defined on a spatial domain.
ARAGAO-COSTA, E. R., Wang, Hongyun, ADDAS-ZANATA, Salvador, Forest, M. G., CARABALLO, T., Mañas, Manuel, Qi, Yuanwei, TAL, Fabio Armando, Stirnemann, A., LANGA, J. A., M Gregory Forest, Wang, ZA, Politi, A., Li, Y., Oppo, Gian-Luca, Doebeli, Michael, Alexander Plakhov, Martínez Salazar, Salomé, Alena Aleksenko, Franzosi, R., Winkler, M, Forest, M. Gregory, Roshchina, V., Mañas Baena, Manuel Enrique, Wang, Q., Lorenzo Nicolodi, Cassatella-Contra, Giovanni A, Tempesta, Piergiulio, Carvalho, Alexandre Nolasco, de Laire, André, Politi, Antonio, Hongyun Wang, CARLOS MEJIA MONASTERIO, Gian-Luca Oppo, Giulio Tiozzo, Nakkeeran, K, Tanaka, Kuzanaga, Zhou, Hong, Elmhirst, Toby, Wang, H. Y., Cassatella Contra, Giovanni A, J A Langa, Emilio Musso, Caraballo Garrido, Tomás, Tanaka, Kazunaga, Antonio Politi, Wrzosek, D, NICOLODI, Lorenzo, Martinez, Salome, Hong Zhou, Ma, M, Li, Yi, Plakhov, A., Burbanks, Andrew, Zhang, Tonghua, Tade, Moses, Laire, André de, Roberto Livi, Oppo, G. L., Carlos Mejia-Monasterio, E R Aragão-Costa, Song, Yongli, Langa Rosado, José Antonio, Carlo Carminati, Manoel, Miriam Garcia, Aleksenko, A., Roberts, Mark, Qi, YW, CARVALHO, A. N., Livi, R., Rondoni, Lamberto, Franzosi, Roberto, T Caraballo, Zhou Hong, Musso, Emilio, Broughan, Kevin A., Roberto Franzosi, Hong Zhou, Tiozzo G., E. Musso, Nicolodi L., Aragão Costa, Eder Ritis, CARMINATI, CARLO, Nicolodi, L., Laire, Andrés de, Wang, Qi, Vera Roshchina, Felmer Aichele, Patricio, Qi Wang, A N Carvalho, Stewart, Ian, Livi, Roberto, Felmer, Patricio   +92 more
core   +1 more source

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

FEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell, Cassy Ross, Panagiota S. Filippou, Bruno Silvester Lopes, Dominic Gilchrist, Paul Dean, Kamar Ameen‐Ali, Tora K. Smulders‐Srinivasan, Ahmad A. Khundakar   +8 more
wiley   +1 more source

The numerical computation of connecting orbits in dynamical systems [PDF]

, 1990
Beyn W-J. The numerical computation of connecting orbits in dynamical systems. IMA Journal of Numerical Analysis. 1990;10(3):379-405.Structural changes in dynamical systems are often related to the appearance or disappearance of orbits connecting two ...
Beyn, Wolf-Jürgen
core   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Deep Learning Pose Estimation for Phenotyping of Co‐Occurring Hyperkinetic Movement Disorders

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To explore whether routine outpatient video combined with deep learning‐based pose estimation and clinically interpretable kinematic features can support multi‐label phenotyping of co‐occurring hyperkinetic movement disorders (HMDs).
Laura Cif, Diane Demailly, Gabriella A. Horvàth, Juan Dario Ortigoza Escobar, Nathalie Dorison, Mayté Castro Jiménez, Cécile A. Hubsch, Thomas Wirth, Gun‐Marie Hariz, Sophie Huby, Morgan Dornadic, Zohra Souei, Muhammad Mushhood Ur Rehman, Simone Hemm, Mehdi Boulaymen, Eduardo M. Moraud, Jocelyne Bloch, Xavier Vasques   +17 more
wiley   +1 more source

Justifying Definitions in Mathematics—Going Beyond Lakatos [PDF]

, 2009
This paper addresses the actual practice of justifying definitions in mathematics. First, I introduce the main account of this issue, namely Lakatos's proof-generated definitions.
Werndl, Charlotte
core   +1 more source

Physician‐Scientist Pipeline for Pediatric Rheumatology: The Current Landscape and Future Perspectives

Arthritis Care &Research, EarlyView.
Over the past 50 years, the science of pediatric rheumatology has grown exponentially due to an expansion in the understanding of complex rheumatic conditions and a surge in novel targeted therapeutics. Physician‐scientists in the field of pediatric rheumatology have played major roles in these advancements that have improved the care of children ...
Ekemini A. Ogbu, Linda T. Hiraki, Mara L. Becker   +2 more
wiley   +1 more source