A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey +4 more
wiley +1 more source
Fruit resources shape sexual selection processes in a lek mating system. [PDF]
Anderson HL, Cabo J, Karubian J.
europepmc +1 more source
Softness of selection and mating system interact to shape trait evolution under sexual conflict. [PDF]
Li Richter XY, Hollis B.
europepmc +1 more source
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
Mixed mating system and intraspecific variation in lizard pollination of <i>Withania frutescens</i> (L.) Pauquy. [PDF]
Muñoz-Gallego R +3 more
europepmc +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Urbanization Alters Phenology, Mating System Allocation, and Life History of <i>Impatiens capensis</i> (Balsaminaceae) via Trait-Specific Plasticity and Genetic Differentiation. [PDF]
Stanley AM, Ashman TL.
europepmc +1 more source
Depicting the mating system and patterns of contemporary pollen flow in trees of the genus Anadenanthera (Fabaceae). [PDF]
Feres JM +5 more
europepmc +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Unveiling the Genome-Wide Consequences of Range Expansion and Mating System Transitions in Primula vulgaris. [PDF]
Mora-Carrera E +11 more
europepmc +1 more source

