FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
The complex evolution and genomic dynamics of mating-type loci in Cryptococcus and Kwoniella. [PDF]
PLoS BiolCoelho MA +9 more
europepmc +1 more source
Dynamics of transposable element accumulation in the non-recombining regions of mating-type chromosomes in anther-smut fungi. [PDF]
Nat Commun, 2023 Duhamel M +3 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Mating-Type Loci Modulate Pathogenicity and Non-Sexual Development Through Autocrine Pheromone Signalling in the Asexual Fungus Fusarium oxysporum. [PDF]
Mol Plant PatholVitale S +6 more
europepmc +1 more source
Didymella fabae Punith.: mating type occurrence, distribution and phenotyping of the anamorph Ascochyta fabae Speg. in Tunisia. [PDF]
Front Plant Sci, 2023 Omri Ben Youssef N +6 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Terfezia claveryi MAT locus characterization uncovers evolutionary insights about sexual reproduction of Pezizomycetes and reveals mating type dynamics in mycorrhizal plants. [PDF]
MycorrhizaAndreu-Ardil L +7 more
europepmc +1 more source
The Mating Type (MAT) and Virulence of <i>Sporothrix schenckii sensu stricto</i> Isolates Maintained in Culture Collection. [PDF]
Microorganisms, 2023 Barreira T +5 more
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source