Results 51 to 60 of about 502 (138)
Modeling Mechanical Dispersion by Using the Method of Advective Transport Phenomena, a First Step
A new theory for mechanical dispersion describes the spreading of water particles generated by advective flow at sub‐model scale. A representative parameter for modeling, the advective volume shift, is calculated from the distance traveled in the calculation step and stochastic parameters describing the aquifer heterogeneity only and replaces the ...
Willem J. de Lange
wiley +1 more source
Terminus DNA loss occurs in a matP recB mutant.
(A) and (B) Micrographs showing ydeV:: parSpMT1 focus behaviour during growth of matP and matP recB cells. Arrows indicate segregating ydeV:: parSpMT1 foci. Cells that generate a focus-less cell during division are circled with a full white line.
Jean-Michel Desfontaines (4484038) +6 more
core +1 more source
Decoding Urease Inhibition: A Comprehensive Review of Inhibitor Scaffolds
Representative functional groups known for urease inhibition are reviewed within diverse scaffolds using structure–activity analyses to identify features associated with high inhibitory activity. Urease is a metalloenzyme produced by a wide range of organisms and plays a critical role in nitrogen microbial metabolism by catalyzing the hydrolysis of ...
Nuno Martinho, Natália Aniceto
wiley +1 more source
Biomanufacturing Potential of Streamlined Cells
ABSTRACT A series of Escherichia coli streamlined strains was developed by removing the expression of genes encoding extracellular structures and unessential enzymes. The streamlined strains exhibited improved metabolic performance, including lower overflow metabolism and ATP maintenance coefficient, as well as a higher growth rate, compared to their ...
Alvaro R. Lara +10 more
wiley +1 more source
ZapA uses a two‐pronged mechanism to facilitate Z ring formation in Escherichia coli
Abstract The tubulin‐like protein FtsZ assembles into the Z ring that leads to the assembly and activation of the division machinery in most bacteria. ZapA, a widely conserved protein that interacts with FtsZ, plays a pivotal role in organizing FtsZ filaments into a coherent Z ring.
Yuanyuan Cui +10 more
wiley +1 more source
Proteomic analysis of human frontal and temporal cortex using iTRAQ-based 2D LC-MS/MS
Background The human brain is the most complex organ in the body, and it is important to have a better understanding of how the protein composition in the brain regions contributes to the pathogenesis of associated neurological disorders. Methods In this
Long Xu +9 more
doaj +1 more source
Purification and Some Properties of Carboxypeptidase from Black Matpe Seedlings
6日間暗所発芽させた黒緑豆種子よりCPaseを精製し, その酵素化学的性質を検討した。1) 粗酵素液を硫安分画 (30~70%), Sephadex G-150ゲルろ過, DEAE-Sepharoseクロマトグラフィー, SephadexG-150再ゲルろ過, DBAE-Sepharose再クロマトグラフィーおよびTOYOPEARL HW-50Fゲルろ過を行なうことにより, ディスク電気泳動的に均一なCP-ase標品を単離した。収率は7.8%であり比活性の上昇率は110倍であった。ゲルろ過法による分子量は約7万と推定された。2) 精製CPaseのZ-Phe-Ala水解時における至適pHは6.0, 至適温度は50℃であった。室温2時間放置時の安定pH領域は5~8であり, 10分間熱処理時の耐熱性は50℃
TSURUSHIIN, Shigeko, FUKUBA, Hiroyasu
openaire +2 more sources
Changes of Proteins and Enzyme Activity in Black Matpe Organs during Germination.
ブラックマッペの発芽にともなう各部位のタンパク質の変化を, ゲル濾過法を用い検討した。同時にプロテアーゼインヒビター活性およびプロテアーゼ活性を調べ, タンパク質の変化と酵素活性の変動との関連性をも検討した。さらに7日発芽体の部位別の, 遊離アミノ酸組成を調べたところ以下のことが明らかとなった。1) 発芽にともない, 子葉のタンパク質量は減少したが, 胚軸では, 発芽とともにわずかではあるが増加した。2) 未発芽豆の子葉と胚芽のタンパク質組成を比較したところ, 三つのピークが認められ, 子葉においてその比率は, 分子量の大きいものから順に, 7: 2: 1, 胚芽では4: 5: 1であった。また貯蔵タンパク質と思われるピーク1 (分子量の最も大きいもの) について ...
YOSHIDA, Keiko +4 more
openaire +2 more sources
PALM imaging of MatP and z-position estimation.
(A) Four representative PALM images for cells expressing MatP-mEos2 in the order of bright-field, ensemble fluorescence, and PALM image. Scale Bars, 500 nm.
Jie Xiao (113647) +5 more
core +1 more source
Aims Insular cortex is a brain region critical for processing of the sensation. Purinergic receptors are involved in the formation of chronic pain. The aim of the present study was to explore the role and mechanism of P2X3 receptors (P2X3Rs) in insular ...
Ping-An Zhang +5 more
doaj +1 more source

