Results 1 to 10 of about 489 (129)

MukBEF-dependent chromosomal organization in widened Escherichia coli [PDF]

Frontiers in Microbiology, 2023
The bacterial chromosome is spatially organized through protein-mediated compaction, supercoiling, and cell-boundary confinement. Structural Maintenance of Chromosomes (SMC) complexes are a major class of chromosome-organizing proteins present throughout
Cees Dekker, Aleksandre Japaridze, Jacob W J Kerssemakers   +2 more
exaly   +10 more sources

DNA sliding and loop formation by E. coli SMC complex: MukBEF [PDF]

Biochemistry and Biophysics Reports, 2022
SMC (structural maintenance of chromosomes) complexes share conserved architectures and function in chromosome maintenance via an unknown mechanism. Here we have used single-molecule techniques to study MukBEF, the SMC complex in Escherichia coli.
Man Zhou
doaj   +8 more sources

MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation [PDF]

Nature Communications, 2016
MukBEF, the bacterial structural maintenance of chromosomes complex, is known to associate with origins of replication and topoisomerase IV. Here the authors show an association of MukBEF with MatP and replication termination regions, important for ...
Anjana Badrinarayanan, Sophie Nolivos, Katarzyna Zawadzka   +2 more
exaly   +8 more sources

The Localization and Action of Topoisomerase IV in Escherichia coli Chromosome Segregation Is Coordinated by the SMC Complex, MukBEF [PDF]

Cell Reports, 2015
The type II topoisomerase TopoIV, which has an essential role in Escherichia coli chromosome decatenation, interacts with MukBEF, an SMC (structural maintenance of chromosomes) complex that acts in chromosome segregation.
Mathew Stracy, Achillefs Kapanidis, Katarzyna Zawadzka   +2 more
exaly   +7 more sources

Mechanism of DNA capture by the MukBEF SMC complex and its inhibition by a viral DNA mimic [PDF]

Cell
Ring-like structural maintenance of chromosome (SMC) complexes are crucial for genome organization and operate through mechanisms of DNA entrapment and loop extrusion. Here, we explore the DNA loading process of the bacterial SMC complex MukBEF. Using cryoelectron microscopy (cryo-EM), we demonstrate that ATP binding opens one of MukBEF's three ...
Mark Dillingham, Jan Lowe, Frank Bürmann   +2 more
exaly   +5 more sources

Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation [PDF]

Nature Communications, 2021
E. coli MukBEF is an SMC complex that plays key roles in chromosome organization-segregation. Here the authors show that the interaction between MukBEF and the Acyl Carrier Protein (AcpP) is essential for MukBEF activity in vitro (ATPase) and in vivo.
Josh P. Prince, Jani R. Bolla, Gemma L. M. Fisher, Jarno Mäkelä, Marjorie Fournier, Carol V. Robinson, Lidia K. Arciszewska, David J. Sherratt   +7 more
doaj   +6 more sources

Competitive binding of MatP and topoisomerase IV to the MukB hinge domain [PDF]

eLife, 2021
Structural Maintenance of Chromosomes (SMC) complexes have ubiquitous roles in compacting DNA linearly, thereby promoting chromosome organization-segregation.
Gemma LM Fisher, Jani R Bolla, Karthik V Rajasekar, Jarno Mäkelä, Rachel Baker, Man Zhou, Josh P Prince, Mathew Stracy, Carol V Robinson, Lidia K Arciszewska, David J Sherratt   +10 more
doaj   +5 more sources

Principles of bacterial genome organization, a conformational point of view. [PDF]

Mol Microbiol
Chromosome conformation capture coupled with deep sequencing (Hi‐C) has revealed the diversity of genome architectures across bacterial species, characterizing new chromosome structural layers and the activities of factors involved in their organization.
Ponndara S, Kortebi M, Boccard F, Bury-Moné S, Lioy VS.   +4 more
europepmc   +2 more sources

Physical models of bacterial chromosomes. [PDF]

Mol Microbiol
Connecting experimental observations to biophysical models can give rise to new insights on bacterial chromosome organization. Simple polymer models explore how physical constraints affect bacterial chromosome organization; bottom‐up mechanistic models connect these constraints to their underlying causes; and data‐driven models are inferred directly ...
Harju J, Broedersz CP.
europepmc   +2 more sources

Activity of MukBEF for chromosome management in E. coli and its inhibition by MatP

ELife
Structural maintenance of chromosomes (SMC) complexes share conserved structures and serve a common role in maintaining chromosome architecture. In the bacterium Escherichia coli, the SMC complex MukBEF is necessary for rapid growth and the accurate ...
Stéphane Duigou, Frédéric Boccard
exaly   +3 more sources