Results 31 to 40 of about 489 (129)
MukBEF-dependent chromosomal organization in widened Escherichia coli
, 2022 Abstract The bacterial chromosome is spatially organized through protein-mediated compaction, supercoiling, and cell-boundary confinement. Structural Maintenance of Chromosomes (SMC) complexes are a major class of chromosome-organizing proteins present throughout all domains of life. Here, we study the role of the
Japaridze A +4 more
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The MaoP/maoS Site-Specific System Organizes the Ori Region of the E. coli Chromosome into a Macrodomain. [PDF]
PLoS Genetics, 2016 The Ori region of bacterial genomes is segregated early in the replication cycle of bacterial chromosomes. Consequently, Ori region positioning plays a pivotal role in chromosome dynamics. The Ori region of the E.
Michèle Valens +2 more
doaj +1 more source
BMC Research Notes, 2020 Objective Structural maintenance of chromosomes (SMC) proteins are key players in chromosome dynamics in all types of organisms. The so-called condensin subfamily is essential for chromosome condensation in eukaryotic cells, as is the bacterial SMC ...
Tobias Knust, Peter L. Graumann
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Focal localization of MukBEF condensin on the chromosome requires the flexible linker region of MukF [PDF]
The FEBS Journal, 2009 Condensin complexes are the key mediators of chromosome condensation. The MukB–MukE–MukF complex is a bacterial condensin, in which the MukB subunit forms a V‐shaped dimeric structure with two ATPase head domains. MukE and MukF together form a tight complex, which binds to the MukB head via the C‐terminal winged‐helix domain (C‐WHD) of MukF. One of the
Shin, HC, Lim, JH, Woo, JS, Oh, BH
openaire +3 more sources
MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin
eLife, 2018 The Escherichia coli SMC complex, MukBEF, acts in chromosome segregation. MukBEF shares the distinctive architecture of other SMC complexes, with one prominent difference; unlike other kleisins, MukF forms dimers through its N-terminal domain.
Katarzyna Zawadzka +6 more
doaj +1 more source
Molecular Microbiology, Volume 114, Issue 5, Page 839-856, November 2020., 2020 The rod‐shaped Myxococcus xanthus cells have a unique chromosome organization whereby the chromosome occupies the central part of cells leaving large subpolar regions devoid of DNA. We show that three systems contribute to chromosome segregation and organization in M.
Deepak Anand +2 more
wiley +1 more source
ATP-Induced Shrinkage of DNA with MukB Protein and the MukBEF Complex of Escherichia coli [PDF]
Journal of Bacteriology, 2008 ABSTRACT Fluorescence microscopic observation of individual T4 DNA molecules revealed that the MukBEF complex (bacterial condensin) and its subunit, the MukB (a member of the SMC [structural maintenance of chromosomes] superfamily) homodimer, of Escherichia coli markedly shrunk large DNA molecules in the ...
Ning, Chen +7 more
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Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance of Chromosome (SMC) Complex in Live Escherichia coli Bacteria [PDF]
, 2022 MukBEF, a structural maintenance of chromosome (SMC) complex, is an important molecular machine for chromosome organization and segregation in Escherichia coli. Fluorescently tagged MukBEF forms distinct spots (or "foci") composed of molecular assemblies
Badrinarayanan, Anjana, Leake, Mark C
core +1 more source
, 2023 Chromosome condensation and segregation are two essential processes for the transmission of genetic material in all living organisms. Indeed, chromosomes are composed of a DNA molecule and several families of proteins that perform various functions ...
Seba, Mohammed
core +1 more source
Loop-extrusion-mediated plasmid DNA cleavage by the bacterial SMC Wadjet complex. [PDF]
Mol CellStructural maintenance of chromosomes (SMC) complexes play pivotal roles in genome organization and maintenance across all domains of life. In prokaryotes, SMC-family Wadjet complexes structurally resemble the widespread MukBEF but serve a defensive role
Pradhan B +5 more
europepmc +2 more sources