Bacterial Chromosome Organization and Segregation [PDF]
, 2018 If fully stretched out, a typical bacterial chromosome would be nearly 1 mm long, approximately 1,000 times the length of a cell. Not only must cells massively compact their genetic material, but they must also organize their DNA in a manner that is ...
Badrinarayanan, Anjana +2 more
core +1 more source
Using Fluorescence Recovery After Photobleaching (FRAP) to study dynamics of the Structural Maintenance of Chromosome (SMC) complex in vivo [PDF]
, 2016 The SMC complex, MukBEF, is important for chromosome organization and segregation in Escherichia coli. Fluorescently tagged MukBEF forms distinct spots (or 'foci') in the cell, where it is thought to carry out most of its chromosome associated activities.
A Badrinarayanan +21 more
core +2 more sources
A mechanistic understanding of bacterial chromosome organisation [PDF]
, 2023 Chromosomes serve as repositories of genetic information, crucial to the functionality of all living organisms. Bacteria, in their simplicity, offer an ideal model to investigate the intricacies of chromosomal dynamics.
Sadhir, Ismath
core +1 more source
MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin
eLife, 2018 The Escherichia coli SMC complex, MukBEF, acts in chromosome segregation. MukBEF shares the distinctive architecture of other SMC complexes, with one prominent difference; unlike other kleisins, MukF forms dimers through its N-terminal domain.
Katarzyna Zawadzka +6 more
doaj +1 more source
Cell boundary confinement sets the size and position of the E. coli chromosome [PDF]
, 2019 Although the spatiotemporal structure of the genome is crucial to its biological function, many basic questions remain unanswered on the morphology and segregation of chromosomes.
Chaudhuri, Debasish +11 more
core +2 more sources
Molecular Microbiology, Volume 114, Issue 5, Page 839-856, November 2020., 2020 The rod‐shaped Myxococcus xanthus cells have a unique chromosome organization whereby the chromosome occupies the central part of cells leaving large subpolar regions devoid of DNA. We show that three systems contribute to chromosome segregation and organization in M.
Deepak Anand +2 more
wiley +1 more source
Intermittent transport of bacterial chromosomal loci [PDF]
, 2017 The short-time dynamics of bacterial chromosomal loci is a mixture of subdiffusive and active motion, in the form of rapid relocations with near-ballistic dynamics.
Calabrese, Ludovico +3 more
core +2 more sources
Determining cellular CTCF and cohesin abundances to constrain 3D genome models. [PDF]
, 2019 Achieving a quantitative and predictive understanding of 3D genome architecture remains a major challenge, as it requires quantitative measurements of the key proteins involved.
Cattoglio, Claudia +11 more
core +2 more sources
3D Organization of Eukaryotic and Prokaryotic Genomes [PDF]
, 2020 There is a complex mutual interplay between three-dimensional (3D) genome organization and cellular activities in bacteria and eukaryotes. The aim of this thesis is to investigate such structure-function relationships. A main part of this thesis deals
Hofmann, Andreas
core +1 more source
Comparative genomics of Burkholderia multivorans, a ubiquitous pathogen with a highly conserved genomic structure [PDF]
, 2017 The natural environment serves as a reservoir of opportunistic pathogens. A well-established method for studying the epidemiology of such opportunists is multilocus sequence typing, which in many cases has defined strains predisposed to causing infection.
Peeters, Charlotte +5 more
core +3 more sources