Novobiocin Susceptibility of MukBEF-Deficient Escherichia coli Is Combinatorial with Efflux and Resides in DNA Topoisomerases [PDF]
Antimicrobial Agents and Chemotherapy, 2016 ABSTRACT Condensins play a key role in the global organization of bacterial chromosomes. In Escherichia coli , the inactivation of its sole condensin MukBEF induces severe growth defects and renders cells hypersusceptible to novobiocin.
Zoya M, Petrushenko +3 more
openaire +2 more sources
Video_1_MukBEF-dependent chromosomal organization in widened Escherichia coli.AVI
, 2023 The bacterial chromosome is spatially organized through protein-mediated compaction, supercoiling, and cell-boundary confinement. Structural Maintenance of Chromosomes (SMC) complexes are a major class of chromosome-organizing proteins present throughout
Aleksandre Japaridze (1421389) +5 more
core +1 more source
Mechanism of DNA entrapment by the MukBEF SMC complex and its inhibition by a viral DNA mimic
Summary Ring-like structural maintenance of chromosomes (SMC) complexes are crucial for genome organization and operate through mechanisms of DNA entrapment and loop extrusion. Here, we explore the DNA loading process of the bacterial SMC complex MukBEF.
Bürmann F +6 more
europepmc +2 more sources
Capturing chromosome conformation in Crenarchaea
Molecular Microbiology, Volume 123, Issue 2, Page 101-108, February 2025.To fit into cells, chromosomes must be folded. Here we describe how members of the Archaea effect this folding process and how chromosome architecture both influences and is influenced by gene expression. Abstract While there is a considerable body of knowledge regarding the molecular and structural biology and biochemistry of archaeal information ...
Elyza Pilatowski‐Herzing +5 more
wiley +1 more source
, 2021 Abstract Structural Maintenance of Chromosomes (SMC) complexes contribute ubiquitously to chromosome organization-segregation. SMC proteins have a conserved architecture, with a dimerization hinge and an ATPase head domain separated by a long antiparallel intramolecular coiled-coil.
Josh Prince +7 more
openaire +1 more source
Non-random segregation of sister chromosomes by Escherichia coli MukBEF axial cores [PDF]
, 2020 Summary The Escherichia coli structural maintenance of chromosomes complex, MukBEF, forms axial cores to chromosomes that determine their spatio-temporal organization. Here, we show that axial cores direct chromosome arms to opposite poles and generate the translational symmetry ...
Jarno Mäkelä +2 more
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Rok from B. subtilis: Bridging genome structure and transcription regulation
Molecular Microbiology, Volume 123, Issue 2, Page 109-123, February 2025.In our review on the dual role of B. subtilis Rok as genome organizer and gene regulator, we argue that these two functions are in fact two sides of the same coin and that the coupling between them should be taken into account in further research. We also propose a list of characteristics to aid in determining whether a protein belongs to the H‐NS‐like
Amanda M. Erkelens +3 more
wiley +1 more source
Acyl Carrier Protein is essential for MukBEF action in Escherichia coli chromosome organization-segregation [PDF]
, 2021 Abstract Structural Maintenance of Chromosomes (SMC) complexes contribute ubiquitously to chromosome organization-segregation. SMC proteins have a conserved architecture, with a dimerization hinge and an ATPase head domain separated by a long antiparallel intramolecular coiled-coil.
Josh P. Prince +6 more
openaire +1 more source
Data belonging to: "Organization of the Escherichia coli chromosome by a MukBEF axial core."
, 2020 Data sets for "Organization of the Escherichia coli chromosome by a MukBEF axial core". For raw image files not uploaded here (due to size restrictions) ask the corresponding author of the paper.
sherratt, D (via Mendeley Data)
core +1 more source
Image_1_MukBEF-dependent chromosomal organization in widened Escherichia coli.pdf
, 2023 The bacterial chromosome is spatially organized through protein-mediated compaction, supercoiling, and cell-boundary confinement. Structural Maintenance of Chromosomes (SMC) complexes are a major class of chromosome-organizing proteins present throughout
Aleksandre Japaridze (1421389) +5 more
core +1 more source