Results 61 to 70 of about 775 (136)

Capturing chromosome conformation in Crenarchaea

Molecular Microbiology, Volume 123, Issue 2, Page 101-108, February 2025.
To fit into cells, chromosomes must be folded. Here we describe how members of the Archaea effect this folding process and how chromosome architecture both influences and is influenced by gene expression. Abstract While there is a considerable body of knowledge regarding the molecular and structural biology and biochemistry of archaeal information ...
Elyza Pilatowski‐Herzing, Rachel Y. Samson, Naomichi Takemata, Catherine Badel, Peter B. Bohall, Stephen D. Bell   +5 more
wiley   +1 more source

Mutational analysis of MukE reveals its role in focal subcellular localization of MukBEF [PDF]

Molecular Microbiology, 2012
SummaryBacterial condensin MukBEF is essential for global folding of the Escherichia coli chromosome. MukB, a SMC (structural maintenance of chromosome) protein, comprises the core of this complex and is responsible for its ATP‐modulated DNA binding and reshaping activities.
Weifeng, She, Elena, Mordukhova, Hang, Zhao, Zoya M, Petrushenko, Valentin V, Rybenkov   +4 more
openaire   +2 more sources

Rok from B. subtilis: Bridging genome structure and transcription regulation

Molecular Microbiology, Volume 123, Issue 2, Page 109-123, February 2025.
In our review on the dual role of B. subtilis Rok as genome organizer and gene regulator, we argue that these two functions are in fact two sides of the same coin and that the coupling between them should be taken into account in further research. We also propose a list of characteristics to aid in determining whether a protein belongs to the H‐NS‐like
Amanda M. Erkelens, Bert van Erp, Wilfried J. J. Meijer, Remus T. Dame   +3 more
wiley   +1 more source

Studies of Single-Molecule Dynamics in Microorganisms [PDF]

, 2019
Fluorescence microscopy is one of the most extensively used techniques in the life sciences. Considering the non-invasive sample preparation, enabling live-cell compliant imaging, and the specific fluorescence labeling, allowing for a specific visualization
Turkowyd, Bartosz Daniel
core   +1 more source

Chromosome organization by a conserved condensin-ParB system in the actinobacterium Corynebacterium glutamicum [PDF]

, 2020
Higher-order chromosome folding and segregation are tightly regulated in all domains of life. In bacteria, details on nucleoid organization regulatory mechanisms and function remain poorly characterized, especially in non-model species.
Bramkamp, Marc, Böhm, Kati, Giacomelli, Giacomo, Imhof, Axel, Koszul, Romain, Marbouty, Martial, Schmidt, Andreas   +6 more
core   +3 more sources

Acyl Carrier Protein is essential for MukBEF action in Escherichia coli chromosome organization-segregation

, 2021
Abstract Structural Maintenance of Chromosomes (SMC) complexes contribute ubiquitously to chromosome organization-segregation. SMC proteins have a conserved architecture, with a dimerization hinge and an ATPase head domain separated by a long antiparallel intramolecular coiled-coil.
Josh Prince, Jani Bolla, Gemma Fisher, Jarno Makela, Majorie Fournier, Carol Robinson, Lidia Arciszewska, David Sherratt   +7 more
openaire   +1 more source

Mechanism of DNA entrapment by the MukBEF SMC complex and its inhibition by a viral DNA mimic

SummaryRing-like structural maintenance of chromosomes (SMC) complexes are crucial for genome organization and operate through mechanisms of DNA entrapment and loop extrusion. Here, we explore the DNA loading process of the bacterial SMC complex MukBEF. Using electron cryomicroscopy (cryo-EM), we demonstrate that ATP binding opens one of MukBEF’s three
Bürmann F, Clifton B, Koekemoer S, Wilkinson OJ, Kimanius D, Dillingham MS, Löwe J.   +6 more
europepmc   +2 more sources

Bacterial chromatin proteins, transcription, and DNA topology: Inseparable partners in the control of gene expression

Molecular Microbiology, Volume 122, Issue 1, Page 81-112, July 2024.
In this review article, the diverse mechanisms by which bacterial chromatin proteins influence and are influenced by transcription and DNA supercoiling are discussed. Abstract DNA in bacterial chromosomes is organized into higher‐order structures by DNA‐binding proteins called nucleoid‐associated proteins (NAPs) or bacterial chromatin proteins (BCPs ...
Christine M. Hustmyer, Robert Landick
wiley   +1 more source

Insights Into the Chromatin Structure of Thermoplasma volcanium: Archaeal HU Regulates Alba‐Mediated DNA Compaction

Archaea, Volume 2024, Issue 1, 2024.
Archaeal species encode a diversity of chromatin proteins that play distinct roles in genome compaction. Although our understanding of the individual proteins has been growing, their contributions to higher‐order folding in the archaeal chromosome remain to be explored. In this study, we investigated the chromatin structure of Thermoplasma volcanium, a
Kirk Amerigo B. Aycardo, Eloise I. Prieto, Stefan Spring   +2 more
wiley   +1 more source

Rules and Exceptions: The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes [PDF]

, 2020
: The segregation of newly replicated chromosomes in bacterial cells is a highly coordinated spatiotemporal process. In the majority of bacterial species, a tripartite ParAB-parS system, composed of an ATPase (ParA), a DNA-binding protein (ParB), and its
Bartosik, Aneta A, Jagura-Burdzy, Grazyna, Kawalek, Adam, Wawrzyniak, Paweł   +3 more
core   +1 more source