Focal localization of MukBEF condensin on the chromosome requires the flexible linker region of MukF [PDF]
The FEBS Journal, 2009 Condensin complexes are the key mediators of chromosome condensation. The MukB–MukE–MukF complex is a bacterial condensin, in which the MukB subunit forms a V‐shaped dimeric structure with two ATPase head domains. MukE and MukF together form a tight complex, which binds to the MukB head via the C‐terminal winged‐helix domain (C‐WHD) of MukF. One of the
Shin, HC, Lim, JH, Woo, JS, Oh, BH
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Are SMC complexes loop extruding factors? Linking theory with fact [PDF]
, 2019 The extreme length of chromosomal DNA requires organizing mechanisms to both promote functional genetic interactions and ensure faithful chromosome segregation when cells divide.
Akai +102 more
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Bacillus Subtilis SMC Complexes Juxtapose Chromosome Arms as They Travel from Origin to Terminus [PDF]
, 2017 Structural maintenance of chromosomes (SMC) complexes play critical roles in chromosome dynamics in virtually all organisms, but how they function remains poorly understood.
Brandão, Hugo B. +4 more
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Independent mobility of proteins and lipids in the plasma membrane of Escherichia coli [PDF]
, 2016 Biotechnology and Biological Sciences Research Council. Grant Number: BB/E009571, Oxford Centre for Integrative Systems Biology (OCISB), Engineering and Physical Science Research Council, Royal Society, Hertford College ...
Leake, MC +6 more
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Acyl Carrier Protein is essential for MukBEF action inEscherichia colichromosome organization-segregation [PDF]
, 2021 AbstractStructural Maintenance of Chromosomes (SMC) complexes contribute ubiquitously to chromosome organization-segregation. SMC proteins have a conserved architecture, with a dimerization hinge and an ATPase head domain separated by a long antiparallel intramolecular coiled-coil.
Josh P. Prince +6 more
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High-resolution definition of the Vibrio cholerae essential gene set with hidden Markov model–based analyses of transposon-insertion sequencing data [PDF]
, 2014 The coupling of high-density transposon mutagenesis to high-throughput DNA sequencing (transposon-insertion sequencing) enables simultaneous and genome-wide assessment of the contributions of individual loci to bacterial growth and survival.
Chao, Michael C. +6 more
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The ABCs of SMC proteins: two-armed ATPases for chromosome condensation, cohesion and repair [PDF]
, 2002 The first draft of the human genome sequence was reported a year ago. It may be a good time to remind ourselves that the genetic information encoded in the ∼3000-Mb sequence is stored not only in the public or private databases but also in the tiny space
Hirano, T.
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ATP-Induced Shrinkage of DNA with MukB Protein and the MukBEF Complex of Escherichia coli [PDF]
Journal of Bacteriology, 2008 ABSTRACT Fluorescence microscopic observation of individual T4 DNA molecules revealed that the MukBEF complex (bacterial condensin) and its subunit, the MukB (a member of the SMC [structural maintenance of chromosomes] superfamily) homodimer, of Escherichia coli markedly shrunk large DNA molecules in the ...
Ning, Chen +7 more
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Structural and biochemical studies of the S. cerevisiae condensin complex [PDF]
, 2020 The Structural Maintenance of Chromosomes (SMC) complex family forms an essential part of the genome’s organisational machinery in all domains of life. In eukaryotes condensin is one such complex that mediates the packaging of DNA for segregation during ...
Cawood, Christopher
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Transient non-specific DNA binding dominates the target search of bacterial DNA-binding proteins [PDF]
, 2021 International audienceAbstract The global emergence of drug-resistant bacteria leads to the loss of efficacy of our antibiotics arsenal and severely limits the success of currently available treatments.
Kapanidis, A. +5 more
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