Results 21 to 30 of about 775 (136)

Organization of the Escherichia coli Chromosome by a MukBEF Axial Core [PDF]

Molecular Cell, 2020
Structural maintenance of chromosomes (SMC) complexes organize chromosomes ubiquitously, thereby contributing to their faithful segregation. We demonstrate that under conditions of increased chromosome occupancy of the Escherichia coli SMC complex, MukBEF, the chromosome is organized as a series of loops around a thin (
Jarno Mäkelä, David John Sherratt
exaly   +6 more sources

Transient growth arrest in Escherichia coli induced by chromosome condensation. [PDF]

PLoS ONE, 2013
MukB is a bacterial SMC (structural maintenance of chromosome) protein that regulates the global folding of the Escherichia coli chromosome by bringing distant DNA segments together.
Andrea L Edwards, Dipen P Sangurdekar, Kyeong S Jeong, Arkady B Khodursky, Valentin V Rybenkov   +4 more
doaj   +6 more sources

Bacillus subtilis Smc condenses chromosomes in a heterologous cell system, which is down-regulated by ScpAB [PDF]

BMC Research Notes, 2020
Objective Structural maintenance of chromosomes (SMC) proteins are key players in chromosome dynamics in all types of organisms. The so-called condensin subfamily is essential for chromosome condensation in eukaryotic cells, as is the bacterial SMC ...
Tobias Knust, Peter L. Graumann
doaj   +2 more sources

Self-organised segregation of bacterial chromosomal origins [PDF]

eLife, 2019
The chromosomal replication origin region (ori) of characterised bacteria is dynamically positioned throughout the cell cycle. In slowly growing Escherichia coli, ori is maintained at mid-cell from birth until its replication, after which newly ...
Andreas Hofmann, Jarno Mäkelä, David J Sherratt, Dieter Heermann, Seán M Murray   +4 more
doaj   +2 more sources

Dynamic architecture of the Escherichia coli structural maintenance of chromosomes (SMC) complex, MukBE [PDF]

Nucleic Acids Research, 2019
Ubiquitous Structural Maintenance of Chromosomes (SMC) complexes use a proteinaceous ring-shaped architecture to organize and individualize chromosomes, thereby facilitating chromosome segregation.
Arciszewska, Lidia K., Baker, Rachel, Bolla, Jani, Koczy, O, Rajasekar, KV, Robinson, Carol V., Sherratt, David, Tang, M, Wagner, Florence, Zawadzka, K   +9 more
core   +6 more sources

The SMC Complex MukBEF Recruits Topoisomerase IV to the Origin of Replication Region in Live Escherichia coli [PDF]

mBio, 2014
The Escherichia coli structural maintenance of chromosome (SMC) complex, MukBEF, and topoisomerase IV (TopoIV) interact in vitro through a direct contact between the MukB dimerization hinge and the C-terminal domain of ParC, the catalytic subunit of ...
Emilien Nicolas, Amy L. Upton, Stephan Uphoff, Olivia Henry, Anjana Badrinarayanan, David Sherratt   +5 more
doaj   +6 more sources

Physical models of bacterial chromosomes. [PDF]

Mol Microbiol
Connecting experimental observations to biophysical models can give rise to new insights on bacterial chromosome organization. Simple polymer models explore how physical constraints affect bacterial chromosome organization; bottom‐up mechanistic models connect these constraints to their underlying causes; and data‐driven models are inferred directly ...
Harju J, Broedersz CP.
europepmc   +2 more sources

MukBEF, a Chromosomal Organizer [PDF]

Microbial Physiology, 2014
Global folding of bacterial chromosome requires the activity of condensins. These highly conserved proteins are involved in various aspects of higher-order chromatin dynamics in a diverse range of organisms. Two distinct superfamilies of condensins have been identified in bacteria.
Valentin V, Rybenkov, Viridiana, Herrera, Zoya M, Petrushenko, Hang, Zhao   +3 more
openaire   +2 more sources

MukBEF-dependent chromosomal organization in widened Escherichia coli

, 2022
AbstractThe bacterial chromosome is spatially organized through protein-mediated compaction, supercoiling, and cell-boundary confinement. Structural Maintenance of Chromosomes (SMC) complexes are a major class of chromosome-organizing proteins present throughout all domains of life.
Japaridze A, van Wee R, Gogou C, Kerssemakers JWJ, Dekker C.   +4 more
europepmc   +2 more sources

Loop-extrusion-mediated plasmid DNA cleavage by the bacterial SMC Wadjet complex. [PDF]

Mol Cell
Structural maintenance of chromosomes (SMC) complexes play pivotal roles in genome organization and maintenance across all domains of life. In prokaryotes, SMC-family Wadjet complexes structurally resemble the widespread MukBEF but serve a defensive role
Pradhan B, Deep A, König J, Baaske MD, Corbett KD, Kim E.   +5 more
europepmc   +3 more sources