Results 11 to 20 of about 489 (129)
Organization of the Escherichia coli Chromosome by a MukBEF Axial Core [PDF]
Molecular Cell, 2020 Structural maintenance of chromosomes (SMC) complexes organize chromosomes ubiquitously, thereby contributing to their faithful segregation. We demonstrate that under conditions of increased chromosome occupancy of the Escherichia coli SMC complex, MukBEF, the chromosome is organized as a series of loops around a thin (
David Sherratt, Jarno Mäkelä
exaly +8 more sources
The SMC Complex MukBEF Recruits Topoisomerase IV to the Origin of Replication Region in Live
mBio, 2014 The Escherichia coli structural maintenance of chromosome (SMC) complex, MukBEF, and topoisomerase IV (TopoIV) interact in vitro through a direct contact between the MukB dimerization hinge and the C-terminal domain of ParC, the catalytic subunit of ...
Emilien Nicolas +5 more
doaj +8 more sources
Intersubunit and intrasubunit interactions driving the MukBEF ATPase [PDF]
Journal of Biological Chemistry, 2022 MukBEF, a structural maintenance of chromosome-like protein complex consisting of an ATPase, MukB, and two interacting subunits, MukE and MukF, functions as the bacterial condensin. It is likely that MukBEF compacts DNA via an ATP hydrolysis-dependent DNA loop-extrusion reaction similar to that demonstrated for the yeast structural maintenance of ...
Rupesh Kumar +2 more
exaly +5 more sources
Nonrandom segregation of sister chromosomes by <i>Escherichia coli</i> MukBEF. [PDF]
Proc Natl Acad Sci U S A, 2021 Significance Circular chromosomes in rod-shaped bacteria exist inside a cell in two distinct configurations, “transverse” and “longitudinal,” relative to the long cell axis, with chromosomal loci occupying specific cellular locations in both cases. Bacteria with longitudinal chromosome organization (e.g.,
Mäkelä J, Uphoff S, Sherratt DJ.
europepmc +6 more sources
Activity of MukBEF for chromosome management in E. coli and its inhibition by MatP. [PDF]
Elife, 2023 Structural maintenance of chromosomes (SMC) complexes share conserved structures and serve a common role in maintaining chromosome architecture. In the bacterium Escherichia coli , the SMC complex MukBEF is necessary for rapid growth and the accurate segregation and positioning of the ...
Seba M, Boccard F, Duigou S.
europepmc +6 more sources
Role of MukBEF in Escherichia coli chromosome organization [PDF]
, 2011 In E. coli cells, spatial chromosome organization reflects the genetic map, with the origin located at mid-cell and left and right replichores in either cell half. In contrast to the eukaryotic cell cycle, chromosome segregation occurs progressively as replication proceeds.
Badrinarayanan, Anjana, Sherratt, David
core +4 more sources
Cryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites [PDF]
Molecular Cell, 2021 Summary The ring-like structural maintenance of chromosomes (SMC) complex MukBEF folds the genome of Escherichia coli and related bacteria into large loops, presumably by active DNA loop extrusion.
Jan Lowe, Frank Bürmann, Jason W Chin
exaly +5 more sources
A folded conformation of MukBEF and Cohesin [PDF]
Nature Structural & Molecular Biology, 2018 Abstract Structural maintenance of chromosomes (SMC)-kleisin complexes organize chromosomal DNAs in all domains of life, where they have key roles in chromosome segregation, DNA repair and regulation of gene expression. They function through topological entrapment and active translocation of DNA, but the underlying conformational ...
Frank Bürmann +9 more
openaire +6 more sources
Dissecting the role of MukF in the chromosome organisation by Escherichia coli MukBEF [PDF]
, 2019 The organisation and segregation of chromosomes from all domains of life are mediated by the architecturally conserved Structural Maintenance of Chromosomes (SMC) proteins and their accessory factors. In Escherichia coli, the distantly related complex MukBEF retains the distinctive tripartite ring structure that has been proposed to topologically ...
Rachel Baker, Baker, Rachel
openaire +3 more sources
Self-organised segregation of bacterial chromosomal origins
eLife, 2019 The chromosomal replication origin region (ori) of characterised bacteria is dynamically positioned throughout the cell cycle. In slowly growing Escherichia coli, ori is maintained at mid-cell from birth until its replication, after which newly ...
Andreas Hofmann +4 more
doaj +2 more sources