Results 81 to 90 of about 989,555 (231)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Cryo-Gel embedding compound for renal biopsy biobanking

Scientific Reports, 2019
Optimal preservation and biobanking of renal tissue is vital for good diagnostics and subsequent research. Optimal cutting temperature (OCT) compound is a commonly used embedding medium for freezing tissue samples. However, due to interfering polymers in
Malou L. H. Snijders, Marina Zajec, Laurens A. J. Walter, Remco M. A. A. de Louw, Monique H. A. Oomen, Shazia Arshad, Thierry P. P. van den Bosch, Lennard J. M. Dekker, Michail Doukas, Theo M. Luider, Peter H. J. Riegman, Folkert J. van Kemenade, Marian C. Clahsen-van Groningen   +12 more
doaj   +1 more source

A combined linkage and exome sequencing analysis for electrocardiogram parameters in the Erasmus Rucphen Family study

Frontiers in Genetics, 2016
Electrocardiogram (ECG) measurements play a key role in the diagnosis and prediction of cardiac arrhythmias and sudden cardiac death. ECG parameters, such as the PR, QRS, and QT intervals, are known to be heritable and genome-wide association studies ...
Claudia Tamar Silva, Claudia Tamar Silva, Claudia Tamar Silva, Irina Zorkoltseva, Najaf Amin, Ayse Demirkan, Ayse Demirkan, Elisabeth Maria van Leeuwen, Jan Kors, Marten van den Berg, Bruno Stricker, Bruno Stricker, Bruno Stricker, André Uitterlinden, Anatoly Kirichenko, Jacqueline C.M. Witteman, Rob Willemsen, Ben Oostra, Ben Oostra, Tatiana Axenovich, Cornelia Marja Van Duijn, Cornelia Marja Van Duijn, Aaron Isaacs, Aaron Isaacs   +23 more
doaj   +1 more source

Ant-colony-based multiuser detection for multifunctional-antenna-array-assisted MC DS-CDMA systems

, 2008
A novel Ant Colony Optimization (ACO) based Multi-User Detector (MUD) is designed for the synchronous Multi-Functional Antenna Array (MFAA) assisted Multi-Carrier Direct-Sequence Code-Division Multiple-Access (MC DS-CDMA) uplink (UL), which supports both
Xu, C., Yang, L-L., Hanzo, L., Hu, B.
core   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

A Dosimetric Comparison Study for Blood Irradiation Employing Different Medium and Algorithms in Clinical Linear Accelerator

Journal of Blood Medicine
Sarath S Nair,1 Jyothi Nagesh,1 Shambhavi C,2 Anshul Singh,1 Shirley Lewis,1 Umesh Velu,1 Deepika Chenna3 1Department of Radiation Oncology, Kasturba Medical College Manipal MAHE, Manipal, Karnataka, India; 2Department of Medical Radiation Physics ...
Nair SS, Nagesh J, C S, Singh A, Lewis S, Velu U, Chenna D   +6 more
doaj  

Enamel matrix derivative has no effect on the chondrogenic differentiation of mesenchymal stem cells

Frontiers in Bioengineering and Biotechnology, 2014
Background: Treatment of large bone defects due to trauma, tumour resection, or congenital abnormalities is challenging. Bone tissue engineering using mesenchymal stem cells (MSCs) represents a promising treatment option.
Lisanne C Groeneveldt, Callie eKnuth, Janneke eWitte-Bouma, Fergal J O'Brien, Eppo B Wolvius, Eric eFarrell   +5 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Gene length corrected trimmed mean of M-values (GeTMM) processing of RNA-seq data performs similarly in intersample analyses while improving intrasample comparisons

BMC Bioinformatics, 2018
Background Current normalization methods for RNA-sequencing data allow either for intersample comparison to identify differentially expressed (DE) genes or for intrasample comparison for the discovery and validation of gene signatures.
Marcel Smid, Robert R. J. Coebergh van den Braak, Harmen J. G. van de Werken, Job van Riet, Anne van Galen, Vanja de Weerd, Michelle van der Vlugt-Daane, Sandra I. Bril, Zarina S. Lalmahomed, Wigard P. Kloosterman, Saskia M. Wilting, John A. Foekens, Jan N. M. IJzermans, on behalf of the MATCH study group, John W. M. Martens, Anieta M. Sieuwerts   +15 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source