Results 191 to 200 of about 57,512 (227)
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Regulation of the MDCK Cell Tight Junction
Journal of Membrane Biology, 1998The sodium flux across individual tight junctions (TJ) of low-resistance MDCK cell monolayers grown on glass coverslips was determined as a measure of paracellular permeability. Increases in perfusate glucose concentration from 5 to 25 mM decreased tight junction Na permeability. This permeability decrease was not specific as nonmetabolizable analogues
O N, Kovbasnjuk +2 more
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1985
The use of monolayers of MDCK cells as a model system for natural transporting epithelia created the necessity of learning about their ion-translocating mechanisms. These mechanisms have been analyzed directly by measuring unidirectional fluxes of tracers, and indirectly through their consequences on electrical parameters and blister-forming activity ...
S. Fernández-Castelo +4 more
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The use of monolayers of MDCK cells as a model system for natural transporting epithelia created the necessity of learning about their ion-translocating mechanisms. These mechanisms have been analyzed directly by measuring unidirectional fluxes of tracers, and indirectly through their consequences on electrical parameters and blister-forming activity ...
S. Fernández-Castelo +4 more
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1992
In polarized cells the plasma membrane is divided into apical and basolateral domains. Membrane is internalized from both these domains and the internalized components can follow several different routes, for example, they can be transported to the opposite domain of the cell (transcytosis), routed to lysosomes or recycled back to the same domain ...
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In polarized cells the plasma membrane is divided into apical and basolateral domains. Membrane is internalized from both these domains and the internalized components can follow several different routes, for example, they can be transported to the opposite domain of the cell (transcytosis), routed to lysosomes or recycled back to the same domain ...
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Perforated MDCK cells support intracellular transport.
The EMBO Journal, 1987We have developed a method for perforating the plasma membrane of MDCK cells while retaining cellular functions. A nitrocellulose acetate filter was applied to the apical side of cells, grown on a glass coverslip, and allowed to dry. Segments of the apical plasma membrane adhered to the filter and were detached from the cell layer by shearing when the ...
K, Simons, H, Virta
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Tight junction formation in cultured epithelial cells (MDCK)
The Journal of Membrane Biology, 1985Synthesis and assembly of tight junctions are studied in monolayers of MDCK cells plated at a density sufficient for confluence, allowed to attach for 1 hr, and transferred to fresh media without cells containing or not Ca2+. 20 hr later, while monolayers with Ca2+ have fully developed junctions that confer an electrical resistance across of 346 +/- 51
L, Gonzalez-Mariscal +2 more
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Anion channels for amino acids in MDCK cells
American Journal of Physiology-Cell Physiology, 1992Large losses of amino acids by diffusion were previously observed in Madin-Darby canine kidney (MDCK) cells during volume regulation. Also, an outward rectifying anion channel was activated. Because this channel was not selective among anions, it was suggested that it could be permeable to amino acids.
U, Banderali, G, Roy
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Polarized trafficking of thyrocyte proteins in MDCK cells
Molecular and Cellular Endocrinology, 2002Recent studies suggest striking similarities between polarized protein sorting in thyrocytes and MDCK epithelial cells, including apical trafficking of thyroglobulin (Tg), thyroid peroxidase, and aminopeptidase N; as well as basolateral targeting of heparan sulfate proteoglycans, thrombospondin 1 (TSP1), type 1 5'-deiodinase, sodium-potassium ATPase ...
Xiaoqing, Zhang +3 more
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Giant MDCK cells: a novel expression system.
Methods in enzymology, 1990This chapter describes a technique by which an epithelial cell line can be used for expression experiments. Basically, cells are harvested from a subconfluent monolayer and fused to form giant cells. For expression experiments the giant cells have to be identified first, then injected individually, and, finally, 24 hr after microinjection, re ...
Oberleithner, H. +5 more
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Safety of MDCK Cell Culture-Based Influenza Vaccines
Future Microbiology, 2011After more than 60 years, the conventional production of influenza vaccines employing fertilized chicken eggs has reached its limits - both in terms of temporal flexibility and vaccine production volume. This problem is compounded by the fact that the pandemic-driven situation in 2009 has roughly doubled the overall vaccine demand.
Jens-Peter, Gregersen +3 more
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Na + -independent proton secretion in MDCK-C11 cells
Pfl�gers Archiv European Journal of Physiology, 2000In this work we studied the proton secretion mechanisms in recently cloned MDCK-C11 cells. We measured intracellular pH (pHi) in monolayers grown on permeable filters, using the pH-sensitive probe BCECF and an inverted epifluorescence microscope. The cells have a basal pHi of 7.20+/-0.01 (n=136) and after an acid-releasing NH4Cl pulse pHi recovered at ...
R, Fernandez +2 more
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