SLC35A2 Deficiency Promotes an Epithelial-to-Mesenchymal Transition-like Phenotype in Madin–Darby Canine Kidney Cells [PDF]
Cells, 2022 In mammalian cells, SLC35A2 delivers UDP–galactose for galactosylation reactions that take place predominantly in the Golgi lumen. Mutations in the corresponding gene cause a subtype of a congenital disorder of glycosylation (SLC35A2-CDG).
Magdalena Kot +7 more
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Canid herpesvirus 1 Preferentially Infects Polarized Madin-Darby Canine Kidney Cells from the Basolateral Surface [PDF]
Viruses, 2022 Canid herpesvirus 1 (CHV-1) infects polarized canine epithelia. Herein, we present our initial work characterizing CHV-1 infection of Madin-Darby canine kidney (MDCK) cells that were polarized on trans-wells.
Mohamed Eisa +2 more
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Epithelial sheet folding induces lumen formation by Madin-Darby canine kidney cells in a collagen gel. [PDF]
PLoS ONE, 2014 Lumen formation is important for morphogenesis; however, an unanswered question is whether it involves the collective migration of epithelial cells. Here, using a collagen gel overlay culture method, we show that Madin-Darby canine kidney cells migrated ...
Sumire Ishida +6 more
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Kaohsiung Journal of Medical Sciences, 2015 This study explored the effect of deltamethrin, a pesticide, on free Ca2+ concentration [Ca2+]i, viability, and apoptosis in Madin–Darby canine kidney (MDCK) canine renal tubular cells.
Fang-Jin Liu +10 more
doaj +3 more sources
Inhibitory actions by ibandronate sodium, a nitrogen-containing bisphosphonate, on calcium-activated potassium channels in Madin–Darby canine kidney cells [PDF]
Toxicology Reports, 2015 The nitrogen-containing bisphosphonates used for management of the patients with osteoporosis were reported to influence the function of renal tubular cells.
Sheng-Nan Wu +4 more
doaj +2 more sources
Transcytosis of polymeric immunoglobulin a in polarized Madin-Darby canine kidney cells. [PDF]
Methods Mol Biol, 2008 The transcytotic pathway allows for the bidirectional transport of endocytosed solutes, lipids, and proteins between the two membrane domains of polarized epithelial cells while maintaining the functional integrity of the epithelial tissue. A method is described to measure basolateral-to-apical transcytosis of immunoglobulin A (IgA) in polarized Madin ...
Oztan A, Rondanino C, Apodaca G.
europepmc +4 more sources
Interlaboratory Variability in the Madin–Darby Canine Kidney Cell Proteome
Molecular Pharmaceutics, 2023 Madin-Darby canine kidney (MDCK) cells are widely used to study epithelial cell functionality. Their low endogenous drug transporter protein levels make them an amenable system to investigate transepithelial permeation and drug transporter protein activity after their transfection.
Matthew D. Harwood +11 more
openaire +3 more sources
Induction of Epithelial-mesenchymal Transition in MDCK II Cells
Bio-Protocol, 2021 Epithelial-mesenchymal transition (EMT) is a reversible process of epithelial cell transdifferentiation into a mesenchymal cell, that enables initiation of cell migration.
Agnieszka Pastula, Richard Lundmark
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MethodsX, 2020 CRISPR-Cas9 gene editing has made it possible to specifically edit genes in a myriad of target cells. Here, a method for isoform-specific editing and clonal selection in Madin-Darby canine kidney (MDCK) epithelial cells is described in detail.
James M. Readler +3 more
doaj +1 more source
Endocytosis in filter-grown Madin-Darby canine kidney cells. [PDF]
The Journal of cell biology, 1989 In this paper, we have characterized the apical and basolateral endocytic pathways of epithelial MDCK cells grown on filters. The three-dimensional organization of the endocytic compartments was analyzed by confocal microscopy after internalization of a fluorescent fluid-phase marker from either side of the cell layer.
Bomsel, M. +4 more
openaire +5 more sources