Results 21 to 30 of about 19,777 (180)

Involvement of Intracellular Ca2+ in the Regulatory Volume Decrease After Hyposmotic Swelling in MDCK Cells

Journal of Pharmacological Sciences, 2007
We examined the source of Ca2+ involved in the volume regulation of Madin-Darby canine kidney (MDCK) cells with confocal microscopy and fluoroprobes. Hyposmosis induced a transient increase in cell volume, as well as cytoplasmic Ca2+, which peaked at 3 ...
Naoko Iida-Tanaka, Iyuki Namekata, Megumi Kaneko, Miku Tamura, Toru Kawanishi, Ryu Nakamura, Koki Shigenobu, Hikaru Tanaka   +7 more
doaj   +1 more source

Substrate Stiffness Determines the Establishment of Apical-Basal Polarization in Renal Epithelial Cells but Not in Tubuloid-Derived Cells

Frontiers in Bioengineering and Biotechnology, 2022
Mechanical guidance of tissue morphogenesis is an emerging method of regenerative medicine that can be employed to steer functional kidney architecture for the purpose of bioartificial kidney design or renal tissue engineering strategies.
Maria J. Hagelaars, Maria J. Hagelaars, Fjodor A. Yousef Yengej, Fjodor A. Yousef Yengej, Marianne C. Verhaar, Maarten B. Rookmaaker, Sandra Loerakker, Sandra Loerakker, Carlijn V. C. Bouten, Carlijn V. C. Bouten   +9 more
doaj   +1 more source

Lixazinone Stimulates Mitogenesis of Madin-Darby Canine Kidney Cells

Experimental Biology and Medicine, 2006
Polycystic kidney diseases (PKD) are characterized by excessive proliferation of renal tubular epithelial cells, development of fluid-filled cysts, and progressive renal insufficiency. cAMP inhibits proliferation of normal renal tubular epithelial cells but stimulates proliferation of renal tubular epithelial cells derived from patients with PKD. Madin-
Jingfei, Cheng, Michael A, Thompson, Henry J, Walker, Catherine E, Gray, Gina M, Warner, Wei, Zhou, Joseph P, Grande   +6 more
openaire   +2 more sources

A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells

eLife, 2020
Selective protein distribution on distinct plasma membranes is important for epithelial cell function. To date, how proteins are directed to specific epithelial cell surface is not fully understood. Here we report a conserved DSSDE motif in LDL-receptor (
Ce Zhang, Yue Chen, Shijin Sun, Yikai Zhang, Lina Wang, Zhipu Luo, Meng Liu, Liang Dong, Ningzheng Dong, Qingyu Wu   +9 more
doaj   +1 more source

Different Infectivity of Swine Enteric Coronaviruses in Cells of Various Species

Pathogens
Swine enteric coronaviruses (SECoVs), including porcine deltacoronavirus (PDCoV), transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), and swine acute diarrhea syndrome coronavirus (SADS-CoV), have caused high mortality in ...
Zhongyuan Li, Yunyan Chen, Liang Li, Mei Xue, Li Feng   +4 more
doaj   +1 more source

Sonic hedgehog is basolaterally sorted from the TGN and transcytosed to the apical domain involving Dispatched-1 at Rab11-ARE

Frontiers in Cell and Developmental Biology, 2022
Hedgehog proteins (Hhs) secretion from apical and/or basolateral domains occurs in different epithelial cells impacting development and tissue homeostasis.
Lisette Sandoval, Mariana Labarca, Mariana Labarca, Claudio Retamal, Claudio Retamal, Paula Sánchez, Juan Larraín, Alfonso González, Alfonso González, Alfonso González   +9 more
doaj   +1 more source

In vitro potency test for evaluation of Clostridium perfringens type D epsilon toxoid

Arquivos do Instituto Biológico
The control of enterotoxemia caused by the epsilon toxin, produced by Clostridium perfringenstype D, is based on vaccination with epsilon toxoid. The potency test for this immunogen is conducted using seroneutralization in mice.
Felipe Masiero Salvarini, Zélia Ines Portela Lobato, Prhiscylla Sadanã Pires, Rodrigo Otávio Silveira Silva, Guilherme Guerra Alves, Pedro Lúcio Lithg Pereira, Francisco Carlos Faria Lobato   +6 more
doaj   +1 more source

cfa-miR-143 Promotes Apoptosis via the p53 Pathway in Canine Influenza Virus H3N2-Infected Cells

Viruses, 2017
MicroRNAs regulate multiple aspects of the host response to viral infection. This study verified that the expression of cfa-miR-143 was upregulated in vivo and in vitro by canine influenza virus (CIV) H3N2 infection. To understand the role of cfa-miR-143
Pei Zhou, Liqing Tu, Xi Lin, Xiangqi Hao, Qingxu Zheng, Weijie Zeng, Xin Zhang, Yun Zheng, Lifang Wang, Shoujun Li   +9 more
doaj   +1 more source

Regulation of epithelial migration by epithelial cell adhesion molecule requires its Claudin-7 interaction domain. [PDF]

PLoS ONE, 2018
Epithelial cell adhesion molecule (EpCAM) is a glycoprotein on the surface of epithelial cells that is essential for intestinal epithelial integrity and expressed at high levels in many epithelial derived cancers and circulating tumor cells. Here we show
Angela I M Barth, Honesty Kim, Ingmar H Riedel-Kruse   +2 more
doaj   +1 more source

The assembly of the major desmosome glycoproteins of Madin‐Darby canine kidney cells

FEBS Letters, 1989
Madin‐Darby canine kidney (MDCK) cells are unable to form desmosomes when cultured in low‐calcium medium ([Ca2+]<0.1 meq./l), but can be induced to do so by raising the calcium to physiological concentrations (1–2 meq./l). We have previously demonstrated that this block correlated with increased desmosomal protein turnover.
Penn, Elizabeth J., Hobson, Christine, Rees, David A., Magee, Anthony I.   +3 more
openaire   +2 more sources