Results 31 to 40 of about 19,777 (180)

Microlith Formation In Vitro by Madin Darby Canine Kidney (MDCK) Cells [PDF]

International Journal of Urology, 1996
Background: The mechanism of renal stone genesis as well as the location of stone crystal formation in the kidney remains unclear. Possible sites of stone generation are either in the tubular lumen or tubular cell.Methods: We cultured Madin Darby canine kidney (MDCK), LLC‐PK1 and Magen Krebs Niigata‐28 (MKN‐28) cells in DMEM + 10% FBS medium in a well ...
S, Kageyama, Y, Ohtawara, K, Fujita, T, Watanabe, T, Ushiyama, K, Suzuki, Y, Naito, K, Kawabe   +7 more
openaire   +2 more sources

Design, Synthesis, and Biological Evaluation of EdAP, a 4′-Ethynyl-2′-Deoxyadenosine 5′-Monophosphate Analog, as a Potent Influenza a Inhibitor

Molecules, 2019
Influenza A viruses leading to infectious respiratory diseases cause seasonal epidemics and sometimes periodic global pandemics. Viral polymerase is an attractive target in inhibiting viral replication, and 4′-ethynyladenosine, which has been ...
Toshifumi Takeuchi, Nongluk Sriwilaijaroen, Ayako Sakuraba, Ei Hayashi, Shinji Kamisuki, Yasuo Suzuki, Hiroshi Ohrui, Fumio Sugawara   +7 more
doaj   +1 more source

Characterization of Zn2+ transport in Madin-Darby canine kidney cells

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2003
The aim of this study was to characterize the mechanism implicated in Zn(2+) transport in MDCK cells. Trace elements such as Zn(2+), Cd(2+) or Cu(2+) induced MDCK cell depolarization at the micromolar level as demonstrated by bis-oxonol fluorescence and whole-cell patch experiments.
Ducoudret, Olivier, Barbier, Olivier, Tauc, Michel, Fuchs, Marianne, Poujeol, Philippe   +4 more
openaire   +2 more sources

The Centre for Chemical Sensors and Chemical Information Technology (CCS) in Micro- and Nanotechnology

CHIMIA, 2004
A selection of the most challenging projects in micro- and nanotechnology tackled at CCS during the last few years are discussed. The ultimate challenge was to localize voltage-gated and ligand-gated ion channels on membranes of living cells by ...
Ursula E. Spichiger-Keller, Prisca Schär-Zammarettia   +1 more
doaj   +1 more source

A Qualitative Change in the Transcriptome Occurs after the First Cell Cycle and Coincides with Lumen Establishment during MDCKII Cystogenesis

iScience, 2020
Summary: Madin-Darby canine kidney II (MDCKII) cells are widely used to study epithelial morphogenesis. To better understand this process, we performed time course RNA-seq analysis of MDCKII 3D cystogenesis, along with polarized 2D cells for comparison ...
Tianfang Wang, Sang-Ho Kwon, Xiao Peng, Severine Urdy, Zefu Lu, Robert J. Schmitz, Stephen Dalton, Keith E. Mostov, Shaying Zhao   +8 more
doaj   +1 more source

Role of fibronectin deposition in cystogenesis of Madin-Darby canine kidney cells [PDF]

Kidney International, 1999
Madin-Darby canine kidney (MDCK) cells cultured within collagen I gel exhibit clonal growth and form spherical multicellular cysts. The cyst-lining epithelial cells are polarized with the basolateral surface in contact with the collagen gel and the apical surface facing the lumen.
Jiang, Si-Tse, Chiang, Huei-Ching, Cheng, Min-Hsiung, Yang, Tzi-Peng, Chuang, Woei-Jer, Tang, Ming-Jer   +5 more
openaire   +2 more sources

Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan. [PDF]

PLoS ONE, 2015
A/Narita/1/2009 (A/N) was the first H1N1 virus from the 2009 pandemic (H1pdm) to be isolated in Japan. To better understand and predict the possible development of this virus strain, the effect of passaging A/N was investigated in Madin-Darby canine ...
Akira Ainai, Hideki Hasegawa, Masatsugu Obuchi, Takato Odagiri, Makoto Ujike, Masayuki Shirakura, Eri Nobusawa, Masato Tashiro, Hideki Asanuma   +8 more
doaj   +1 more source

Real‐Time and Non‐Invasive Detection of Respiratory Viral Infections Using an Intelligent Odor Monitoring System (IOMS)

Advanced Science, EarlyView.
An intelligent odor monitoring system integrates an IVC animal model, gas sensor array, real‐time resistance readout, and machine‐learning analysis to continuously monitor infection‐associated odor changes. The platform captures longitudinal sensor responses, distinguishes infected from healthy states, and supports early‐stage respiratory viral ...
Yajie Shen, Weifeng Yuan, Long Li, Yucheng Zheng, Kenan Liu, Hegeng Li, Hua‐Yao Li, Yongliang Zhao, Binzhou Ying, Lanpeng Guo, Wenjian Zhang, Shu‐Ming Kuo, Zirui Zhang, Yufan Deng, Bohan Yin, Zhaocheng Luo, Ke Xu, Huan Liu   +17 more
wiley   +1 more source

Development of an Enhanced High-Yield Influenza Vaccine Backbone in Embryonated Chicken Eggs

Vaccines, 2023
Vaccination is an efficient approach to preventing influenza virus infections. Recently, we developed influenza A and B virus vaccine backbones that increased the yield of several vaccine viruses in Madin–Darby canine kidney (MDCK) and African green ...
Lizheng Guan, Jihui Ping, Tiago J. S. Lopes, Shufang Fan, Robert Presler, Gabriele Neumann, Yoshihiro Kawaoka   +6 more
doaj   +1 more source

PROTAC‐Engineered Protein/DNA Nanoantigen is a Potent Booster for Dendritic Cell Vaccines in Cancer Immunotherapy

Advanced Science, EarlyView.
A PROTAC‐functionalized protein/DNA‐integrated nanoantigen platform is developed to enhance dendritic cell vaccine potency. By combining a VHL‐recruiting peptide‐modified antigen with a sustained expression plasmid within CaCO3 nanoparticles, this system amplifies cross‐presentation, promotes DC maturation, and elicits robust Th1 and CTL responses ...
Peng Liu, Qiang Chen, Zhe Zhou, Yuekang Xu, Jinyao Li   +4 more
wiley   +1 more source