Results 51 to 60 of about 19,777 (180)

Advances in Serum-Free Suspension Culture Technology for Animal Cells and Their Applications

Vaccines
Serum-free suspension culture technology for animal cells involves the division and proliferation of cells in serum-free medium as single cells or cell clusters within shaking flasks or bioreactors.
Wenna Ji, Ziyi Chen, Jinyu Zhou, Xinyu Yue, Zilin Qiao, Jiamin Wang   +5 more
doaj   +1 more source

Development of the evaluation system for barrier functions of engineered epithelial lumens

Regenerative Therapy, 2016
We have investigated the effects of a diameter of engineered epithelial lumen on cellar architectures and a barrier function. For this investigation, we have developed a system to evaluate the barrier function of engineered epithelial lumens. To test the
Kazuya Furusawa, Takeomi Mizutani, Naoki Sasaki   +2 more
doaj   +1 more source

SNHG15 Positively Regulates Influenza Virus Infection Through Its Association With miR‐153 and RABL2A

Journal of Medical Virology, Volume 98, Issue 8, August 2026.
ABSTRACT Influenza viruses cause seasonal epidemics, and current vaccines do not fully protect against them. This study focuses on long non‐coding RNAs (lncRNAs), which are RNA molecules that do not code for proteins but play crucial roles in various biological processes. We identified 17 lncRNAs altered by the influenza virus.
Samuel Pushparaj, Kishore Vaddadi, Chaoqun Huang, Yurong Liang, Dao Xu, Gayan Bamunuarachchi, Prince Jhandai, Keerthana Santhosh, Elizabeth Varghese, Lin Liu   +9 more
wiley   +1 more source

Site-Specific Glycosylation Profiling of Protein Subunit and Inactivated Virus Vaccines

Vaccines
Background/Objectives: Glycosylation can affect vaccine antigen structure and function, making site-specific glycan characterization relevant to antigen quality and comparability.
Zachary C. Goecker, Meghan C. Burke, Yi Liu, Yuri A. Mirokhin, Sergey L. Sheetlin, Guanghui Wang, Dmitrii V. Tchekhovskoi, Xiaoyu Yang, Stephen E. Stein   +8 more
doaj   +1 more source

Environmental hyperosmolality regulates phospholipid biosynthesis in the renal epithelial cell line MDCK

Journal of Lipid Research, 2013
Hyperosmolality is a key signal for renal physiology. On the one hand, it contributes to the differentiation of renal medullary structures and to the development of the urinary concentrating mechanism. On the other, it is a stress factor.
Cecilia I. Casali, Karen Weber, Nicolás O. Favale, María C. Fernández Tome   +3 more
doaj   +1 more source

Pharmacokinetic Drug–Drug Interactions of Imlunestrant in Healthy Female Participants of Nonchildbearing Potential

Clinical and Translational Science, Volume 19, Issue 8, August 2026.
ABSTRACT Imlunestrant is a next‐generation, oral, selective estrogen receptor degrader approved for treating adults with estrogen receptor‐positive, human epidermal growth factor receptor 2‐negative, ESR1‐mutated advanced/metastatic breast cancer. Imlunestrant is metabolized partly by cytochrome P450 (CYP)3A4 oxidation and conjugation with glucuronic ...
Amita Datta‐Mannan, Elaine Shanks, Eunice Yuen, Vivian Rodriguez Cruz, Xuejing Aimee Wang, Stephen David Hall   +5 more
wiley   +1 more source

Preclinical immunogenicity and safety evaluation of MDCK cell-derived quadrivalent influenza virus subunit vaccine

Frontiers in Immunology
To address the limitations of egg-based influenza vaccine production, serum-free suspension culture of Madin-Darby Canine Kidney cells (MDCK) has emerged as a promising alternative.
Shuang Li, Shuang Li, Jialin Lv, Jialin Lv, Xuejiao Li, Xuejiao Li, Yue Yang, Yue Yang, Yimeng Yan, Yimeng Yan, Lina Sui, Lina Sui, Yehong Wu   +12 more
doaj   +1 more source

Rab10 is Involved in Basolateral Transport in Polarized Madin–Darby Canine Kidney Cells [PDF]

Traffic, 2006
The sorting of newly synthesized membrane proteins to the cell surface is an important mechanism of cell polarity. To identify more of the molecular machinery involved, we investigated the function of the small GTPase Rab10 in polarized epithelial Madin–Darby canine kidney cells. We find that GFP‐tagged Rab10 localizes primarily to
Schuck, S., Gerl, M., Ang, A., Manninen, A., Keller, P., Mellman, I., Simons, K.   +6 more
openaire   +4 more sources

Functional Blood‐Brain Barrier Crossing by Biomimetic M13 Phage Vectors for Targeted Neuronal Delivery

Advanced Healthcare Materials, Volume 15, Issue 28, 24 July 2026.
This study investigates the M13 bacteriophage as a biomimetic nanovector capable of crossing in vitro models of the blood–brain barrier. By exploiting peculiar transcellular pathways, M13 avoids lysosomal degradation and preserves its structural integrity and functionality.
Silvia Vercellino, Lucia Pappagallo, Francesca De Chirico, Luca Boselli, Hazem Ahmed, Luca Spagnoletti, Giovanni Pittaluga, Paolo Emidio Costantini, Matteo Calvaresi, Pier Paolo Pompa, Alberto Danielli, Fabio Benfenati, Valentina Castagnola   +12 more
wiley   +1 more source

Chlorpromazine Induces Basolateral Aquaporin-2 Accumulation via F-Actin Depolymerization and Blockade of Endocytosis in Renal Epithelial Cells

Cells, 2020
We previously showed that in polarized Madin–Darby canine kidney (MDCK) cells, aquaporin-2 (AQP2) is continuously targeted to the basolateral plasma membrane from which it is rapidly retrieved by clathrin-mediated endocytosis.
Richard Bouley, Naofumi Yui, Abby Terlouw, Pui W. Cheung, Dennis Brown   +4 more
doaj   +1 more source