Results 11 to 20 of about 32,238 (217)

Proteoglycans in polarized epithelial Madin-Darby canine kidney cells [PDF]

Biochemical Journal, 1995
Madin-Darby canine kidney (MDCK) cells were cultured on polycarbonate filters to study the synthesis and sorting of proteoglycans in polarized epithelial cells. Two strains of MDCK cells were used. MDCK I cells resemble distal tubule epithelial cells, and MDCK II cells share some characteristics with proximal tubule cells.
K, Svennevig, K, Prydz, S O, Kolset
openaire   +2 more sources

Production of isoform-specific knockdown/knockout Madin–Darby canine kidney epithelial cells using CRISPR/Cas9

MethodsX, 2020
CRISPR-Cas9 gene editing has made it possible to specifically edit genes in a myriad of target cells. Here, a method for isoform-specific editing and clonal selection in Madin-Darby canine kidney (MDCK) epithelial cells is described in detail.
James M. Readler, Amal S. AlKahlout, Abimbola O. Kolawole, Katherine J.D.A. Excoffon   +3 more
doaj   +1 more source

Cloning, Prokaryotic Soluble Expression, and Analysis of Antiviral Activity of Two Novel Feline IFN-ω Proteins

Viruses, 2020
Cats are becoming more popular as household companions and pets, forming close relationships with humans. Although feline viral diseases can pose serious health hazards to pet cats, commercialized preventative vaccines are lacking.
Xiaona Wang, Fengsai Li, Meijing Han, Shuo Jia, Li Wang, Xinyuan Qiao, Yanping Jiang, Wen Cui, Lijie Tang, Yijing Li, Yi-Gang Xu   +10 more
doaj   +1 more source

Metabolomics profiling reveals differences in proliferation between tumorigenic and non-tumorigenic Madin-Darby canine kidney (MDCK) cells [PDF]

PeerJ, 2023
Background Madin-Darby canine kidney (MDCK) cells are a cellular matrix in the production of influenza vaccines. The proliferation rate of MDCK cells is one of the critical factors that determine the vaccine production cycle.
Na Sun, Yuchuan Zhang, Jian Dong, Geng Liu, Zhenbin Liu, Jiamin Wang, Zilin Qiao, Jiayou Zhang, Kai Duan, Xuanxuan Nian, Zhongren Ma, Xiaoming Yang   +11 more
doaj   +2 more sources

Aberrant Polycystin-1 Expression Results in Modification of Activator Protein-1 Activity, whereas Wnt Signaling Remains Unaffected [PDF]

, 2004
Polycystin-1, the polycystic kidney disease 1 gene product, has been implicated in several signaling complexes that are known to regulate essential cellular functions. We investigated the role of polycystin-1 in Wnt signaling and activator protein-1 (
Arnould, Bhunia, Duyndam, Giles, Grotewold, Huan, Kim, Kimberling, Kugoh, Loghman-Adham, Löhning, Morin, Moser, Muto, Newby, Ouwens, Parnell, Peters, Pritchard, Rivat, Rodova, Saadi-Kheddouci, Scheffers, Shaulian, Smits, Sorenson, Spaargaren, Streets, Sutters, Trudel, Upadhya, van Adelsberg, van Dam, van Dam, van Dam, van Dam, van de Wetering, van de Wetering   +37 more
core   +21 more sources

Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells. [PDF]

The Journal of cell biology, 1987
To study the intracellular transport of newly synthesized sphingolipids in epithelial cells we have used a fluorescent ceramide analog, N-6[7-nitro-2,1,3-benzoxadiazol-4-yl] aminocaproyl sphingosine (C6-NBD-ceramide; Lipsky, N. G., and R. E. Pagano, 1983, Proc. Natl. Acad. Sci. USA, 80:2608-2612) as a probe. This ceramide was readily taken up by filter-
van Meer, G., Stelzer, E.H., Wijnaendts van Resandt, R.W., Simons, K.   +3 more
openaire   +3 more sources

Ouabain promotes claudin-1, -2, and -4 autophagic degradation through oxidative stress and AMPK activation in MDCK cells

Autophagy Reports, 2023
Epithelial cells transport substances through the cellular and paracellular pathways. The last one depends on tight junctions, particularly on claudins, the family of integral membrane proteins responsible for the permeability and selectivity of these ...
Jessica P. Campos-Blázquez, Catalina Flores-Maldonado, Juan M. Gallardo, José Bonilla-Delgado, Alan A. Pedraza-Ramírez, Octavio López-Méndez, Enoc M. Cortés-Malagón, Rubén G. Contreras   +7 more
doaj   +1 more source

TRAF3 deficiency in MDCK cells improved sensitivity to the influenza A virus

Heliyon, 2023
Tumor necrosis factor receptor-associated factor 3 (TRAF3), an adaptor protein, has significant and varying effects on immunity depending on cell types. The role of TRAF3 in Madin-Darby Canine Kidney Epithelial (MDCK) cell resistance to influenza A virus
Yang Le, Jiayou Zhang, Zheng Gong, Zhegang Zhang, Xuanxuan Nian, Xuedan Li, Daiguan Yu, Ning Ma, Rong Zhou, Guomei Zhang, Bo Liu, Lu Yang, Baiqi Fu, Xiuqin Xu, Xiaoming Yang   +14 more
doaj   +1 more source

Autophagy induction regulates influenza virus replication in a time-dependent manner [PDF]

, 2017
Autophagy plays a key role in host defence responses against microbial infections by promoting degradation of pathogens and participating in acquired immunity.
Abdoli, Asghar, Bamdad, Taravat, Feizi, Amir, Feizi, Neda, Fotouhi, Fatemeh, Jamali, Abbas, Mehrbod, Parvaneh, Nasrollahi Nargesabad, Reza, Ollah Jazaeri, Ehsan, Romani, Bizhan, Saleh, Maryam, Shokouhi Targhi, Hadiseh, Soleimanjahi, Hoorieh   +12 more
core   +1 more source

Mechanisms involved in calcium oxalate endocytosis by Madin-Darby canine kidney cells

Brazilian Journal of Medical and Biological Research, 2000
Calcium oxalate (CaOx) crystals adhere to and are internalized by tubular renal cells and it seems that this interaction is related (positively or negatively) to the appearance of urinary calculi.
A.H. Campos, N. Schor
doaj   +1 more source