Results 191 to 200 of about 12,124,912 (231)

In Polarized MDCK Cells Basolateral Vesicles Arise from Clathrin-γ-adaptin–coated Domains on Endosomal Tubules

Journal of Cell Biology, 1998
Human transferrin receptors (TR) and receptors for polymeric immunoglobulins (pIgR) expressed in polarized MDCK cells maintain steady-state, asymmetric distributions on the separate basolateral and apical surfaces even though they are trafficking ...
Clare E. Futter, A. Gibson, E. H. Allchin, S. Maxwell, L. J. Ruddock, Greg Odorizzi, Derrick L. Domingo, I. S. Trowbridge, Colin R. Hopkins   +8 more
semanticscholar   +1 more source

Measuring receptor recycling in polarized MDCK cells

2015
Recycling of proteins such as channels, pumps, and receptors is critical for epithelial cell function. In this chapter we present a method to measure receptor recycling in polarized Madin-Darby canine kidney cells using an iodinated ligand. We describe a technique to iodinate transferrin (Tf), we discuss how (125)I-Tf can be used to label a cohort of ...
Luciana, Gallo, Gerard, Apodaca
openaire   +2 more sources

Electrical Properties of MDCK Cells

1985
The electrical characterization of a transporting epithelium requires information three main aspects: (1) the overall electrical properties across the whole cell layer; (2) the transcellular permeation route; and (3) the paracellular pathway. In the present article we describe assumptions, techniques, efforts, and information collected on these aspects
L. González-Mariscal, L. Borboa, R. López-Vancell, G. Beaty, M. Cereijido   +4 more
openaire   +1 more source

Transfection Alters Ion Transport in MDCK Cells

Journal of Membrane Biology, 1996
In the course of an investigation into the effect of Tamm-Horsfall protein (THP) on ion transport, we performed stable transfection of THP into MDCK cells using the SV40 or the cytomegalovirus (CMV) promoter. As controls, we transfected MDCK cells with an "empty" plasmid containing SV40 or CMV promoter but without THP cDNA.
D M, Kaji, J, Bates, J D, Goyzueta, K, Prasadan, H, Yu, S, Kumar   +5 more
openaire   +2 more sources

SNARE Protein Trafficking in Polarized MDCK Cells

Traffic, 2000
A key feature of polarized epithelial cells is the ability to maintain the specific biochemical composition of the apical and basolateral plasma membrane domains. This polarity is generated and maintained by the continuous sorting of apical and basolateral components in the secretory and endocytic pathways.
M, Steegmaier, K C, Lee, R, Prekeris, R H, Scheller   +3 more
openaire   +2 more sources

Mineralocorticoid-induced membrane proteins in MDCK cells

Molecular and Cellular Endocrinology, 1982
The Madin-Darby canine kidney (MDCK) cell line, which exhibits properties indicative of a distal tubule origin, evidently binds and responds to mineralocorticoid hormones. We investigated the effects of aldosterone and deoxycorticosterone on protein synthesis in MDCK cells grown either in medium supplemented with serum or in a hormonally defined, serum-
M H, Cobb, C P, Yang, D M, Jefferson, E, Pasnikowski, W N, Scott   +4 more
openaire   +2 more sources

Polarity of the Forssman glycolipid in MDCK epithelial cells

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1987
To determine whether epithelial plasma membrane glycolipids are polarized in a manner analogous to membrane proteins, MDCK cells grown on permeable filters were analyzed for the expression of Forssman ceramide pentasaccharide, the major neutral glycolipid in these cells.
G E, Nichols, T, Shiraishi, M, Allietta, T W, Tillack, W W, Young   +4 more
openaire   +2 more sources

The exocyst localizes to the primary cilium in MDCK cells

Biochemical and Biophysical Research Communications, 2004
Primary cilia play a role in the maintenance of tubular epithelial differentiation and ciliary dysfunction can result in abnormal cyst formation, such as occurs in autosomal dominant polycystic kidney disease (ADPKD). We previously showed that the exocyst, an eight-protein complex involved in the biogenesis of polarity from yeast to mammals, is ...
Katherine K, Rogers, Patricia D, Wilson, Richard W, Snyder, Xiaoyu, Zhang, Wei, Guo, Christopher R, Burrow, Joshua H, Lipschutz   +6 more
openaire   +2 more sources

Optimization of trypsins for influenza A/H1N1 virus replication in MDCK SI-6 cells, a novel MDCK cell line

Journal of Virological Methods, 2018
A cell-based vaccine production method for influenza virus may be an effective and more rapid alternative to egg-based systems. For high-yield virus production, the effect of bovine, porcine, fungal, and recombinant trypsins on influenza A/H1N1 virus replication in MDCK SI-6 cells (SI-6 cells), a novel MDCK cell line developed by our research group ...
Viska I, Iskandar, Yutaka, Sasaki, Naoto, Yoshino, Raden Z R, Abubakar, Shigehiro, Sato, Yasushi, Muraki   +5 more
openaire   +2 more sources

Giant MDCK cells: a novel expression system.

Methods in enzymology, 1990
This chapter describes a technique by which an epithelial cell line can be used for expression experiments. Basically, cells are harvested from a subconfluent monolayer and fused to form giant cells. For expression experiments the giant cells have to be identified first, then injected individually, and, finally, 24 hr after microinjection, re ...
Oberleithner, H., Schwab, A., Westphale, H., Schuricht, B., Püschel, B., Koepsell, H.   +5 more
openaire   +2 more sources