Results 101 to 110 of about 6,190,775 (296)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Campaign rallies and political meaning-making

, 2023
Prior research determines whether politicians at rallies make programmatic, clientelist or personalist appeals. We argue that this reductive approach obscures the variety of meaning-making at rallies. We offer a vision of rallies as complex communicative
Gabrielle Lynch (17067013), Nicole Beardsworth (17067010), Daniel Paget (16988883)   +2 more
core   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Making Meaning, Out of Meaning Making

, 2010
Making Meaning, Out of Meaning Making by Alda M. Blakeney This study examines the ways in which three elementary teachers appropriated and implemented a defined literacy curriculum in their classrooms.
Blakeney, Alda Marcia
core   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

What is the normativity of Meaning?

There has been much debate over whether to accept the claim that meaning is normative. One obstacle to making progress in that debate is that it is not always clear what the claim amounts to.
Whiting, Daniel
core   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

The microbiome in human skin aging

FEBS Letters, EarlyView.
Age‐related skin changes encompass the well‐known visible phenotypic alterations, together with microbiome dysbiosis and a series of molecular aging hallmarks. These hallmarks characterize not only a fully stablished aged phenotype but also the skin aging process itself.
Manuel Huerta Arana, Cornelia Wiegand, Omid Omrani, Mario Fabri   +3 more
wiley   +1 more source

A Partnership in Youth-Led Dissemination and Meaning Making

Journal of Participatory Research Methods
Dissemination is a vital and often under looked step towards leveraging research to inform systematic change. And, yet there has been limited scholarship or guidance around the co-creation of dissemination in partnership with community members.
Shareen Springer, Miranda Ryker, Marmy Hernandez-Gonzalez, Olivia Staten, Kennedy Ruybal, Nicolle Marroquin Leon, Raquel Amador, Gretchen Nihill, gabi Garcia, Maya Ostroff, Jared Reynolds, Mariah Kornbluh   +11 more
doaj   +1 more source