Results 151 to 160 of about 465,082 (214)

Burden of heterozygote carriers for autosomal recessive conditions in the Middle East: A study of 14,392 genomes. [PDF]

HGG Adv
Nkrumah E, Al-Mulla H, Bashar A, Soman V, Saad C, Wang J, Darabi H, Yatsenko SA, Rajkovic A, Chandran U, Mbarek H, Aarabi M.   +11 more
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Radiologist workforce challenges and the burden of image interpretation in Ghana: Perspectives of frontline doctors and implications for healthcare delivery. [PDF]

PLOS Glob Public Health
Ofori-Manteaw B, Ofori EK, Dzefi-Tettey K, Ago JL, Angmorterh SK, Acheampong F.   +5 more
europepmc   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Refining prognosis in advanced renal cell carcinoma: international real-world validation of the Meet-URO score in first-line immunotherapy combinations. [PDF]

Oncologist
Rebuzzi SE, Ghose A, Rudman S, Venugopal B, Young K, Frazer RD, Ayodele O, Stares M, O'Carrigan B, Ali W, McGrane J, Jain A, Fiala O, Chauhan V, Michael A, Zarkar A, Kapur G, Charnley N, Afshar M, Vengalil S, Forde C, Brown J, Ürün Y, Bianchini D, Bahl A, Srihari N, Di Costanzo F, Smalley B, Parkes J, Crabb S, Vasudev N, Poprach A, Brown N, James L, Haywood S, Tapia J, Vijay A, Parry J, Cheung M, Mahajan I, Moon NO, Abrol R, Tkadlecová M, Soe YSY, Zargham A, Smith M, Ashley S, Hardy O, Patel G, Tun KK, Johnston E, Sarwer A, Bölek H, Shrestha R, Challapalli A, Meegan J, Anpalakhan S, Buono F, Kolarikova E, Leung DK, Murianni V, Catalano F, Bimbatti D, Buti S, Signori A, Fornarini G, Rescigno P, Teoh JYC, Banna GL.   +68 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Understanding the conditions for inclusive education: A realist evaluation of a French territorial innovation. [PDF]

PLoS One
Ségard E, Aromatario O, Chervin P, Cambon L.   +3 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source