Results 191 to 200 of about 15,825 (230)
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Characterization of Melittin Effects in Synaptosomes
Neurochemical Research, 1997The effects of melittin at increasing concentrations on: [3H]GABA release from mouse brain synaptosomes; on the radioactivity released from [3H]arachidonic acid labeled synaptosomal membranes; on synaptosomes ultrastructure and on the leakage of the cytoplasmic marker, lactate-dehydrogenase (LDH) was investigated.
L, González +3 more
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Interactions of 2,2,2‐trifluoroethanol with melittin
Magnetic Resonance in Chemistry, 2009AbstractMelittin dissolved in 42% trifluoroethanol‐water at pH 2 has been shown to be α‐helical between residues 6 and 12 and between residues 13 and 25, with the two helical regions separated by a bend at the Leu13 residue. The inter‐helix angle was found to be 154 ± 3° at 0 °C and 135 ± 3° at 25 °C.
Robert C, Neuman, John T, Gerig
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Acyl carrier protein interacts with melittin
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990Acyl carrier protein (ACP) from Escherichia coli has been shown to form complexes with melittin, a cationic peptide from bee venom. ACP is a small (Mr 8847), acidic, Ca2(+)-binding protein, which possesses some characteristics resembling those of regulatory Ca2(+)-binding proteins including interaction with melittin. Complexing between melittin and ACP
Ernst-Fonberg, Mary L. +2 more
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Melittin-Binding of Troponin C
The Journal of Biochemistry, 1993Ca(2+)-dependent interaction between skeletal muscle troponin C and a bee venom melittin, which can be regarded as a mimic of the troponin C-binding peptide of troponin I, was investigated. Sephadex gel chromatography revealed that melittin bound to troponin C irrespective of the presence or absence of Ca2+ in 50 mM KCl and 50 mM Tris-HCl, pH 7.5.
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Melittin: An allergen of honeybee venom
Journal of Allergy and Clinical Immunology, 1977The presence of serum IgE antibodies to melittin was tested by the radioallergosorbent test (RAST). Melittin, the principal protein of honeybee venom, was isolated by gel filtration on Sephadex G-75 and covalently bound to cyanogen bromide-activated microcrystalline cellulose.
B R, Paull, J W, Yunginger, G J, Gleich
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The interaction of calmodulin with melittin
Biochemical and Biophysical Research Communications, 1986Studies utilizing the interaction of melittin with the 1-106 fragment of calmodulin, the protection of calmodulin from tryptic digestion by melittin, and the interaction of the carbocyanine dye Stains-all with the calmodulin-melittin complex have indicated that complex formation of calmodulin with melittin involves the alpha-helical connecting bridge ...
C G, Caday, R F, Steiner
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Interaction of melittin with lipid membranes
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1994Interaction of melittin with lipid membranes was studied systematically with respect to its adsorption onto membranes, its effect on membrane leakage and fusion, and micellization at various melittin/lipid ratios. It was found that melittin has a strong affinity for adsorption onto lipid membranes.
S, Ohki +3 more
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Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985
Abstract Conformational changes in melittin during self-association were investigated by monitoring the quenching of fluorescence of the single tryptophan residue. Acrylamide and the monovalent ions, Cs + and I − , quench melittin by a diffusion-controlled, collisional mechanism, as judged by the viscosity dependence of the process.
Steven C. Quay +2 more
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Abstract Conformational changes in melittin during self-association were investigated by monitoring the quenching of fluorescence of the single tryptophan residue. Acrylamide and the monovalent ions, Cs + and I − , quench melittin by a diffusion-controlled, collisional mechanism, as judged by the viscosity dependence of the process.
Steven C. Quay +2 more
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Archives of Biochemistry and Biophysics, 1990
The (Na+ + K+)ATPase is inhibited by the bee venom polypeptide, melittin. KCl and NaCl protect the enzyme from melittin inhibition. Analysis of the K+ and Na+ protection against melittin inhibition suggested a kinetic model which was consistent with slowly reversible melittin binding, and mutually exclusive binding of melittin with K+ and Na ...
J, Cuppoletti, A J, Abbott
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The (Na+ + K+)ATPase is inhibited by the bee venom polypeptide, melittin. KCl and NaCl protect the enzyme from melittin inhibition. Analysis of the K+ and Na+ protection against melittin inhibition suggested a kinetic model which was consistent with slowly reversible melittin binding, and mutually exclusive binding of melittin with K+ and Na ...
J, Cuppoletti, A J, Abbott
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Topology of the calmodulin-melittin complex.
Journal of molecular biology, 1998The topology of the Ca2+-calmodulin-melittin ternary complex has been investigated by a combined strategy which integrates limited proteolysis and cross-linking experiments with mass spectrometric methodologies. The rationale behind the methods is that the interface regions of two interacting proteins are accessible to the solvent in the isolated ...
Scaloni A +6 more
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