Results 61 to 70 of about 920,485 (301)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Separation of Co(II) from Zinc Plant Acidic Thiocyanate Leach Solutions Containing Co(II) and Ni(II) by Solvent Extraction Using Trioctylamine in Toluene

open access: yes, 2011
In this study, a zinc plant purification cake (CINKUR Co. Turkey) was leached with sulphuric acid and an acidic leach solution containing Zn(II), Fe(II), Pb(II), Al(III), Cu(II), Co(II) and Ni(II) ions was obtained.
Kumbasar, Recep Ali, Eyüpoğlu, Volkan
core   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

open access: yesFEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart   +7 more
wiley   +1 more source

Deep Learning to Analyze Spatter and Melt Pool Behavior During Additive Manufacturing

open access: yesMetals
To capture the complex metallic spatter and melt pool behavior during the rapid interaction between the laser and metal material, high-speed cameras are applied to record the laser powder bed fusion process and generate a large volume of image data.
Deepak Gadde, Alaa Elwany, Yang Du
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Melt Pool and Keyhole Behavior Analysis for Deep Penetration Laser Welding [PDF]

open access: yes, 2010
One usually defines the main characteristic of the welding performances of a given laser system by its “penetration curve” that corresponds to the welding depth as a function of the welding speed Vw for a given set of operating parameters.
R Fabbro, Fabbro, R, FABBRO, Rémy
core   +1 more source

Dispersing hydrophilic nanoparticles in hydrophobic polymers: HDPE/ZnO nanocomposites by a novel template-based approach

open access: yeseXPRESS Polymer Letters, 2014
The efficiency of a novel template-based approach for the dispersion of hydrophilic nanoparticles within hydrophobic polymer matrices is investigated.
G. Filippone   +7 more
doaj   +1 more source

Earth Mantle's Isotopic Record of Progressive Chemical Depletion

open access: yesAGU Advances, 2023
Mid ocean ridge basalts are partial melts of mantle rocks that have become incompatible element depleted prior to recent sub‐ridge melting. This prior extent of melt extraction and concomitant incompatible element depletion is often inferred from the ...
C. Sani   +4 more
doaj   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

open access: yesFEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik   +6 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

open access: yesFEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane   +11 more
wiley   +1 more source

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