Results 241 to 250 of about 5,515,438 (264)

An improved macromolecular crowding sensor CRONOS for detection of crowding changes in membrane-less organelles under stressed conditions

Biochemical and Biophysical Research Communications, 2021
Membrane-less organelles (MLOs) formed by liquid-liquid phase separation (LLPS) play pivotal roles in biological processes. During LLPS, proteins and nucleotides are extremely condensed, resulting in changes in their conformation and biological functions. Disturbed LLPS homeostasis in MLOs is thought to associate with fatal diseases such as amyotrophic
Masahiko Kuroda, Satoshi Narumi, Kohsuke Kanekura   +2 more
exaly   +3 more sources

Intrinsically disordered proteins in overcrowded milieu: Membrane-less organelles, phase separation, and intrinsic disorder

Current Opinion in Structural Biology, 2017
Although the cellular interior is crowded with various biological macromolecules, the distribution of these macromolecules is highly inhomogeneous. Eukaryotic cells contain numerous proteinaceous membrane-less organelles (PMLOs), which are condensed liquid droplets formed as a result of the reversible and highly controlled liquid-liquid phase ...
Vladimir Uversky
exaly   +4 more sources

In Aqua Veritas: The Indispensable yet Mostly Ignored Role of Water in Phase Separation and Membrane-less Organelles

Biochemistry, 2018
Despite the common practice of presenting structures of biological molecules on an empty background and the assumption that interactions between biological macromolecules take place within the inert solvent, water represents an active component of various biological processes. This Perspective addresses indispensable, yet mostly ignored, roles of water
Boris Y. Zaslavsky, Vladimir N. Uversky
openaire   +4 more sources

Known types of membrane-less organelles and biomolecular condensates

2023
Vladimir Uversky
exaly   +2 more sources

Protein intrinsic disorder-based liquid–liquid phase transitions in biological systems: Complex coacervates and membrane-less organelles

Advances in Colloid and Interface Science, 2017
It is clear now that eukaryotic cells contain numerous membrane-less organelles, many of which are formed in response to changes in the cellular environment.
Vladimir Uversky
exaly   +2 more sources

Ageing drop by drop: Disturbance of the membrane-less organelle biogenesis as an aging hallmark

Biochemical and Biophysical Research Communications
Despite extensive research, the features associated with the aging phenotype are not all-inclusive and need to be updated on a regular basis to incorporate new findings. We propose to include the dysfunction of membrane-less organelle (MLO) as a new aging hallmark.
Baraa M.G.A. Saqr, Nikolay O. Kotoyants, Semen V. Nesterov, Vladimir D. Manuylov, Guy W. Dayhoff, Alexander V. Fonin, Konstantin K. Turoverov, Irina M. Kuznetsova, Valentin I. Gordeliy, Nikolay S. Ilyinsky, Vladimir N. Uversky   +10 more
openaire   +2 more sources

Reshuffling Overcrowded Milieu: Stress-Induced Reorganization of the Eukaryotic Membrane-Less Organelles

Cells are crowded entities, but the intracellular space represents an inhomogeneously crowded environment, where the concentrations of macromolecules (proteins, nucleic acids, etc.) are not uniformly distributed throughout the cell resulting in regions with different levels of crowding.
Anastasia A, Gavrilova, Eva A, Shmidt, Vladimir N, Uversky, Alexander V, Fonin   +3 more
openaire   +2 more sources

Major structural features of membrane-less organelles

2023
George L. Parra, David S. Libich
openaire   +1 more source

Winding and Tangling. An Initial Phase of Membrane-Less Organelle Formation

2023
Hiroshi Maita, Shinichi Nakagawa
openaire   +1 more source

Mapping Core Components of Membrane-less Organelles in Living Cells by Phase-APEX2 Proximity Labeling

Abstract Membrane-less organelles (MLOs), formed via liquid-liquid phase separation, are crucial for organization of biomacromolecules and cellular processes, yet their dynamic nature challenges compositional analysis.
Zhuo Chen, Yongzuo Chen, Huarun Ding, Miaodan Huang, Weijie Chen, Bin Fu, Mingchao Wang, Lingqiang Zhang, Wei Qin, Pilong Li   +9 more
openaire   +1 more source