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Membrane protein crystallization
Journal of Structural Biology, 2003The need for high-resolution structure information on membrane proteins is immediate and growing. Currently, the only reliable way to get it is crystallographically. The rate-limiting step from protein to structure is crystal production. An overview of the current ideas and experimental approaches prevailing in the area of membrane protein ...
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Membrane-protein crystals for neutron diffraction [PDF]
Neutron macromolecular crystallography (NMX) has the potential to provide the experimental input to address unresolved aspects of transport mechanisms and protonation in membrane proteins. However, despite this clear scientific motivation, the practical challenges of obtaining crystals that are large enough to make NMX feasible have so far been ...
Sørensen, Thomas Lykke-Møller+13 more
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Prospects for membrane protein crystals in NMX [PDF]
Adding hydrogen atoms and protonation states to structures of membrane proteins requires successful implementation of neutron macromolecular crystallography (NMX). This information would significantly increase our fundamental understanding of the transport processes membrane proteins undertake. To grow the large crystals needed for NMX studies requires
Hjorth-Jensen, Samuel John+3 more
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Liquid crystals and cell membranes
Pure and Applied Chemistry, 1966Abstract
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Membrane-Assisted Crystallization Technology
2013The interest for crystallization processes based on membrane technology has steadily increased over recent years. This has primarily been driven by the unique opportunity such processes provide to control supersaturation rates and the influence they exert on the nucleation stage through a proper regulation of morphological and chemico-physical ...
DRIOLI E., CURCIO, EFREM, DI PROFIO G.
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Crystallization of intrinsic membrane proteins [PDF]
After many years of discouraging failures, it is now possible to crystallize the intrinsic membrane proteins and to obtain structural information from diffraction studies on the crystals. The strategy for the crystallization consists of depletion of boundary phospholipids from the protein and complex formation with specific ligands.
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Crystallization of Integral Membrane Proteins
Current Protocols in Protein Science, 2007AbstractOver the last 20 years, the use of X‐ray crystallography has become a viable technique for the structure determination of integral membrane proteins. However, standard crystallizaton protocols must be modified to account for difficulties involved in handling membrane proteins, which arise primarily from having detergent present.
Susan K. Buchanan+2 more
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Liquid Crystal Polymer Membranes
2015Liquid crystals (LCs) combine properties of both liquids and crystals and can be organized in a variety of nanostructured polymer films with a monolithic structure. Therefore, polymer-based LCs are ultimately suited as membrane with an accurately controlled nanoporosity.
Dirk J. Broer+2 more
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Crystallization of membrane proteins
Current Opinion in Structural Biology, 1994Abstract The process of crystallizing membrane proteins is hampered by several factors. Isolating significant quantities of membrane proteins is difficult because they are unstable and their biochemistry is not well understood. Even when the membrane proteins have been solubilized, several variables must be taken into account in order to achieve ...
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State of the Art and Perspectives in Membranes for Membrane Distillation/Membrane Crystallization
2020This chapter addresses the fundamental concepts and the relevant mathematics related to the transport phenomena through porous hydrophobic membranes of interest in MD and MCr. Membrane material properties, configuration, structures, and fabrication techniques are discussed.
C Meringolo+5 more
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