Results 131 to 140 of about 433,130 (308)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

An NMR Approach for Investigating Membrane Protein–Lipid Interactions Using Native Reverse Micelles

Bio-Protocol
Peripheral membrane proteins (PMPs) are a subgroup of membrane-associated proteins that are water-soluble and bind to membranes, often reversibly, to perform their function.
Sara Walters, Brian Fuglestad
doaj   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Mechanisms regulating the acyl chain composition of membrane lipids in S. cerevisiae

, 2011
The goal of the research described in this thesis was to identify the genes/enzymes involved in acyl chain remodeling of the major membrane lipid phosphatidylcholine (PC) in the model eukaryote S. cerevisiae. A database search yielded 75 candidates with (
De Smet, C.H., Sub Membrane Enzymology begr. 01-06-12, Biochemistry of membranes, Membrane Enzymology   +3 more
core  

Purification of structured lipids using SCCO2 and membrane process

, 2015
The aim of this study was to evaluate the combination of supercritical carbon dioxide (SCCO2) and membrane separation technologies in the purification of structured lipids (SLs) previously obtained.
Sarmento, LAV, de Moura, JMLN, Goncalves, LAG, Petrus, JCC   +3 more
core   +1 more source

PROSPECTS OF DOMESTIC MICRO-INGREDIENTS PRODUCTION [PDF]

Техника и технология пищевых производств, 2016
Due to the continuous increase in the volume of different types of food additives application for technological purposes, a deep studying of approaches and decisions on the production and development of raw sources of reproduction is urgent and demanded.
Starovoytova K.V., Terechuk L.V.
doaj  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Interaction of fatty acids and phospholipids with multiple binding sites on the potassium channel KcsA

, 2011
The majority of lipids that interact with a transmembrane protein act as a solvent for the protein, forming a shell around it; these lipids are called annular or boundary lipids.
Bolivar Gonzalez, Juan
core  

Bacterial reaction centers purified with styrene maleic acid copolymer retain native membrane functional properties and display enhanced stability

, 2014
Integral membrane proteins often present daunting challenges for biophysical characterization, a fundamental issue being how to select a surfactant that will optimally preserve the individual structure and functional properties of a given membrane ...
Swainsbury, David J. K., Swainsbury, D.J.K., Scheidelaar, S., David J. K. Swainsbury, Rienk van Grondelle, Jones, Michael R, Scheidelaar, Stefan, Membrane Biochemistry and Biophysics, Jones, Michael R., J. Antoinette Killian, Swainsbury, David J K, Killian, J. Antoinette, Van Grondelle, Rienk, van Grondelle, R., Jones, M.R., Sub Membrane Biochemistry & Biophysics, Killian, J Antoinette, Michael R. Jones, Killian, J.A., Stefan Scheidelaar   +19 more
core   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source