Results 91 to 100 of about 4,442,719 (304)

Factors influencing the solubilization of membrane proteins from Escherichia coli membranes by styrene–maleic acid copolymers [PDF]

, 2020
Styrene–maleic acid (SMA) copolymers are a promising alternative to detergents for the solubilization of membrane proteins. Here we employ Escherichia coli membranes containing KcsA as a model protein to investigate the influence of different ...
Dörr, Jonas M., Sub Membrane Biochemistry & Biophysics, Membrane Biochemistry and Biophysics, Koorengevel, Martijn C., Jahn, Helene, Killian, J. Antoinette, Antoniciello, Federico, Kopf, Adrian H.   +7 more
core   +1 more source

Subtype selective fluorescent ligands based on ICI 118,551 to study the human β2‐adrenoceptor in CRISPR/Cas9 genome‐edited HEK293T cells at low expression levels

Pharmacology Research & Perspectives, 2021
Fluorescent ligand technologies have proved to be powerful tools to improve our understanding of ligand‐receptor interactions. Here we have characterized a small focused library of nine fluorescent ligands based on the highly selective β2‐adrenoceptor ...
Joëlle Goulding, Sarah J. Mistry, Mark Soave, Jeanette Woolard, Stephen J. Briddon, Carl W. White, Barrie Kellam, Stephen J. Hill   +7 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Immunological and biochemical characterization of the major seed oil-body membrane proteins [PDF]

, 1988
The major seed oil-body membrane proteins have been purified from a range of plant species by preparative SDS/PAGE, The purified proteins were used to elicit antibodies in rabbits and mice.
Deborah, Au Mun Yee
core  

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

CryoEM screening with a blotting instrument: quantifying parameters affecting ice thickness using semi-automated image analysis

Methods in Microscopy
Cryo-electron microscopy (cryoEM) grid preparation is one of the bottlenecks in using cryoEM for macromolecular structure determination. Despite significant advancements in the development of blot-free instruments, their high cost limits their widespread
Valimehr Sepideh, Cho Ellie, Brown Hamish G., McMillan Paul J., Hanssen Eric   +4 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Tumor cells ectopically expressing the membrane-bound form of IL-7 develop an antitumor immune response efficiently in a colon carcinoma model

Molecules and Cells
: Various approaches employing cytokines and cytokine gene–modified tumor cells have been explored to induce antitumor responses, yet their widespread application has been limited due to efficacy concerns and adverse effects. In this study, interleukin-7
Hee-Su Shin, Hyejin Kim, Soon-Gyu Kwon, Hayyoung Lee, Jie-Oh Lee, Young Sang Kim   +5 more
doaj   +1 more source