Results 81 to 90 of about 4,442,719 (304)
A dark intermediate in the fluorogenic reaction between tetrazine fluorophores and trans-cyclooctene
Biophysical Reports, 2022 Fluorogenic labeling via bioorthogonal tetrazine chemistry has proven to be highly successful in fluorescence microscopy of living cells. To date, trans-cyclooctene (TCO) and bicyclonyne have been found to be the most useful substrates for live-cell ...
Felix Hild +4 more
doaj +1 more source
Modelling stem cell differentiation related processes—A practical overview for biologists
FEBS Letters, EarlyView.Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar +4 more
wiley +1 more source
Journal of Immunology Research, 2018 Susceptibility to fungal infection is commonly associated with impaired neutrophil responses. To study the mechanisms underlying this association, we investigated neutrophil recruitment to the conducting airway wall after Aspergillus fumigatus conidium ...
Marina A. Shevchenko +10 more
doaj +1 more source
Visualizing Ligand Binding to a GPCR In Vivo Using NanoBRET
iScience, 2018 Summary: The therapeutic action of a drug depends on its ability to engage with its molecular target in vivo. However, current drug discovery strategies quantify drug levels within organs rather than determining the binding of drugs directly to their ...
Diana C. Alcobia +9 more
doaj +1 more source
FEBS Letters, EarlyView.CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens +8 more
wiley +1 more source
Membrane proteins in the outer mebrane of plastids and mitochondria [PDF]
, 2006 Channels of the plastid and mitochondrial outer membranes facilitate the turnover of molecules and ions via these membranes. Although channels have been studied many questions pertaining to the whole diversity of plastid and mitochondrial channels in ...
Ilkavets, Iryna
core +1 more source
GRK Mediates μ-Opioid Receptor Plasma Membrane Reorganization
Frontiers in Molecular Neuroscience, 2019 Differential regulation of the μ-opioid receptor (MOP) has been linked to the development of opioid tolerance and dependence which both limit the clinical use of opioid analgesics.
Arisbel B. Gondin +8 more
doaj +1 more source
Membrane proteins in nanotechnology [PDF]
The Biochemist, 2009 The potential for nanotechnology to transform modern life has been appreciated for several years. Generally, nanotechnology involves objects with at least one dimension of less than 100 nm. Such stuff is abundant in the natural world, and one of the most exciting current areas in nanoscience research is the use of proteins as nanoscale components of ...
openaire +3 more sources
FEBS Letters, EarlyView.The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi +6 more
wiley +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source