Results 51 to 60 of about 9,896,950 (388)

A Non-imaging High Throughput Approach to Chemical Library Screening at the Unmodified Adenosine-A3 Receptor in Living Cells

Frontiers in Pharmacology, 2017
Recent advances in fluorescent ligand technology have enabled the study of G protein-coupled receptors in their native environment without the need for genetic modification such as addition of N-terminal fluorescent or bioluminescent tags.
Maria Augusta Arruda, Maria Augusta Arruda, Maria Augusta Arruda, Leigh A. Stoddart, Leigh A. Stoddart, Karolina Gherbi, Karolina Gherbi, Stephen J. Briddon, Stephen J. Briddon, Barrie Kellam, Stephen J. Hill, Stephen J. Hill   +11 more
doaj   +1 more source

Structural insight into mitochondrial β-barrel outer membrane protein biogenesis

Nature Communications, 2020
The Sorting and Assembly Machinery (SAM) complex folds beta-barrel proteins and inserts them into the mitochondrial outer membrane. Here authors report cryoEM structures of the SAM complex from Myceliophthora thermophila, which reveals a GST-like fold ...
Kathryn A. Diederichs, Xiaodan Ni, Sarah E. Rollauer, Istvan Botos, Xiaofeng Tan, Martin S. King, Edmund R. S. Kunji, Jiansen Jiang, Susan K. Buchanan   +8 more
doaj   +1 more source

Exploring Ligand Binding to Calcitonin Gene-Related Peptide Receptors

Frontiers in Molecular Biosciences, 2021
Class B1 G protein-coupled receptors (GPCRs) are important targets for many diseases, including cancer, diabetes, and heart disease. All the approved drugs for this receptor family are peptides that mimic the endogenous activating hormones.
Giuseppe Deganutti, Silvia Atanasio, Roxana-Maria Rujan, Patrick M. Sexton, Patrick M. Sexton, Denise Wootten, Denise Wootten, Christopher A. Reynolds   +7 more
doaj   +1 more source

Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM

FEBS Letters, EarlyView.
Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley   +1 more source

TLR3 forms a highly organized cluster when bound to a poly(I:C) RNA ligand

Nature Communications, 2022
TLR3 plays a crucial role in anti-viral immune response by recognizing RNAs produced during virus infection. Here, the authors determine a high resolution structure of the activated cluster of TLR3.
Chan Seok Lim, Yoon Ha Jang, Ga Young Lee, Gu Min Han, Hye Jin Jeong, Ji Won Kim, Jie-Oh Lee   +6 more
doaj   +1 more source

Claudin-1 and -2: Novel Integral Membrane Proteins Localizing at Tight Junctions with No Sequence Similarity to Occludin

Journal of Cell Biology, 1998
Occludin is the only known integral membrane protein localizing at tight junctions (TJ), but recent targeted disruption analysis of the occludin gene indicated the existence of as yet unidentified integral membrane proteins in TJ.
M. Furuse, Kohji Fujita, T. Hiiragi, K. Fujimoto, S. Tsukita   +4 more
semanticscholar   +1 more source

Mapping the evolution of mitochondrial complex I through structural variation

FEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin, Tingting Chen, James A. Letts   +2 more
wiley   +1 more source

Transport of Cytoplasmically Synthesized Proteins into the Mitochondria in a Cell Free System from Neurospora crassa [PDF]

, 1977
Synthesis and transport of mitochondrial proteins were followed in a cell-free homogenate of Neurospora crassa in which mitochondrial translation was inhibited. Proteins synthesized on cytoplasmic ribosomes are transferred into the mitochondrial fraction.
Andrews T. M., Bingham R. W., Blair G. E., Davidian N. M., Dobberstein B., Dolken G., Gaitskhoki V. S., Godinot C., Gonzalez-Cadavid N. F., Gonzalez-Cadavid N. F., Grollmann A. P., Hallermayer G., Kadenbach B., Kadenbach B., Kadenbach B., Kellems R. E., Kellems R. E., Kellems R. E., Kellems R. E., Marra E., Milstein C., Morris A., O'Brien R. L., Pfaff E., Redman C. M., Shore G. C., Takanami M., Tata J. R., Weiss H., Wojtczak L., Wojtczak L.   +30 more
core   +1 more source

Plasmodium falciparum gametogenesis essential protein 1 (GEP1) is a transmission‐blocking target

FEBS Letters, EarlyView.
This study shows Plasmodium falciparum GEP1 is vital for activating sexual stages of malarial parasites even independently of a mosquito factor. Knockout parasites completely fail gamete formation even when a phosphodiesterase inhibitor is added. Two single‐nucleotide polymorphisms (V241L and S263P) are found in 12%–20% of field samples.
Frederik Huppertz, Milagros Siebeck Caturelli, Lina S. Lehmann, Florian Kurth, Alexander G. Maier, Kai Matuschewski   +5 more
wiley   +1 more source

Nanoclustering as a dominant feature of plasma membrane organization [PDF]

, 2014
Early studies have revealed that some mammalian plasma membrane proteins exist in small nanoclusters. The advent of super-resolution microscopy has corroborated and extended this picture, and led to the suggestion that many, if not most, membrane ...
Cambi, Alessandra, García-Parajo, María F., Jacobson, Ken, Thomoson, Nancy, Torreño-Piña, Juan A.   +4 more
core   +5 more sources