Results 31 to 40 of about 4,599,169 (256)

Directed Mutational Strategies Reveal Drug Binding and Transport by the MDR Transporters of Candida albicans

Journal of Fungi, 2021
Multidrug resistance (MDR) transporters belonging to either the ATP-Binding Cassette (ABC) or Major Facilitator Superfamily (MFS) groups are major determinants of clinical drug resistance in fungi.
Atanu Banerjee, Jorgaq Pata, Suman Sharma, Brian C. Monk, Pierre Falson, Rajendra Prasad   +5 more
doaj   +1 more source

Spontaneous Suppressors against Debilitating Transmembrane Mutants of CaMdr1 Disclose Novel Interdomain Communication via Signature Motifs of the Major Facilitator Superfamily

Journal of Fungi, 2022
The Major Facilitator Superfamily (MFS) drug:H+ antiporter CaMdr1, from Candida albicans, is responsible for the efflux of structurally diverse antifungals.
Suman Sharma, Atanu Banerjee, Alexis Moreno, Archana Kumari Redhu, Pierre Falson, Rajendra Prasad   +5 more
doaj   +1 more source

Mass spectrometric analysis of pilus assembly, amyloid fibril formation, and membrane proteins in their native state [PDF]

, 2012
Structural analysis of proteins and their complexes is crucial to understanding protein function. This thesis demonstrates the application of mass spectrometry (MS) to the study of pilus assembly on Gram-negative bacteria and amyloid formation in ...
Leney, Aneika Corrine
core   +4 more sources

Structure and function of bacterial dynamin-like proteins [PDF]

, 2012
Membrane dynamics are essential for numerous cellular processes in eukaryotic and prokaryotic cells. In eukaryotic cells, membrane fusion and fission are often catalyzed by large GTPases of the dynamin protein family. These proteins couple GTP hydrolysis
Bramkamp, Marc
core   +1 more source

Import pathways of precursor proteins into mitochondria [PDF]

, 1988
The precursor of porin, a mitochondrial outer membrane protein, competes for the import of precursors destined for the three other mitochondrial compartments, including the Fe/S protein of the bc1- complex (intermembrane space), the ADP/ATP carrier ...
Pfaller, Rupert, Pfanner, Nikolaus, Steger, Heinrich F., Rassow, Joachim, Neupert, Walter   +4 more
core   +1 more source

A Non-imaging High Throughput Approach to Chemical Library Screening at the Unmodified Adenosine-A3 Receptor in Living Cells

Frontiers in Pharmacology, 2017
Recent advances in fluorescent ligand technology have enabled the study of G protein-coupled receptors in their native environment without the need for genetic modification such as addition of N-terminal fluorescent or bioluminescent tags.
Maria Augusta Arruda, Maria Augusta Arruda, Maria Augusta Arruda, Leigh A. Stoddart, Leigh A. Stoddart, Karolina Gherbi, Karolina Gherbi, Stephen J. Briddon, Stephen J. Briddon, Barrie Kellam, Stephen J. Hill, Stephen J. Hill   +11 more
doaj   +1 more source

The second intracellular loop of the yeast Trk1 potassium transporter is involved in regulation of activity, and interaction with 14–3-3 proteins

Computational and Structural Biotechnology Journal, 2023
Potassium is an essential intracellular ion, and a sufficient intracellular concentration of it is crucial for many processes; therefore it is fundamental for cells to precisely regulate K+ uptake and efflux through the plasma membrane.
Jakub Masaryk, Deepika Kale, Pavel Pohl, Francisco J. Ruiz-Castilla, Olga Zimmermannová, Veronika Obšilová, José Ramos, Hana Sychrová   +7 more
doaj   +1 more source

Effect of DFPP on Ferroptosis in Peripheral Blood CD14+ Monocytes of Hyperlipidemic Patients Involving the ACSL4/LPCAT3/ALOX5 Pathway

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction Hyperlipidemia is a major risk factor for atherosclerotic cardiovascular disease (ASCVD). Although statins are the cornerstone of lipid‐lowering therapy, their use may be limited in some patients by adverse effects. This study evaluated the possible impact of non‐drug treatment using double‐filtration plasmapheresis (DFPP) on CD14+
Yun Cheng, Lan Li, Qingmei Yang, Meipeng Wu   +3 more
wiley   +1 more source

Structural insight into mitochondrial β-barrel outer membrane protein biogenesis

Nature Communications, 2020
The Sorting and Assembly Machinery (SAM) complex folds beta-barrel proteins and inserts them into the mitochondrial outer membrane. Here authors report cryoEM structures of the SAM complex from Myceliophthora thermophila, which reveals a GST-like fold ...
Kathryn A. Diederichs, Xiaodan Ni, Sarah E. Rollauer, Istvan Botos, Xiaofeng Tan, Martin S. King, Edmund R. S. Kunji, Jiansen Jiang, Susan K. Buchanan   +8 more
doaj   +1 more source

A nanoluciferase biosensor to investigate endogenous chemokine secretion and receptor binding

iScience, 2021
Summary: Secreted chemokines are critical mediators of cellular communication that elicit intracellular signaling by binding membrane-bound receptors. Here we demonstrate the development and use of a sensitive real-time approach to quantify secretion and
Carl W. White, Laura E. Kilpatrick, Kevin D.G. Pfleger, Stephen J. Hill   +3 more
doaj   +1 more source