Results 51 to 60 of about 10,936,171 (356)

High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach

Bio-Protocol, 2020
Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is
Franziska Heydenreich, Tamara Miljuš, Dalibor Milić, Dmitry Veprintsev   +3 more
doaj   +1 more source

Extragenic Suppression analysis of TS mutations using Sec61p [PDF]

, 2007
During synthesis, secretory and membrane proteins are cotranslationally translocated into the lumen of the endoplasmic reticulum through an aqueous gated channel.
Sterling Smith
core   +2 more sources

Membrane-mediated protein interactions drive membrane protein organization

Nature Communications, 2022
AbstractThe plasma membrane’s main constituents, i.e., phospholipids and membrane proteins, are known to be organized in lipid-protein functional domains and supercomplexes. No active membrane-intrinsic process is known to establish membrane organization.
Yining Jiang, Batiste Thienpont, Vinay Sapuru, Richard K. Hite, Jeremy S. Dittman, James N. Sturgis, Simon Scheuring   +6 more
openaire   +3 more sources

Nonequilibrium Fluctuations, Travelling Waves, and Instabilities in Active Membranes [PDF]

, 1999
The stability of a flexible fluid membrane containing a distribution of mobile, active proteins (e.g. proton pumps) is shown to depend on the structure and functional asymmetry of the proteins. A stable active membrane is in a nonequilibrium steady state
D. Andelman, E. Evans, E. Evans, F. Brochard, F. Maingret, H. Lodish, J. P. Marcerou, J. Prost, J. Prost, J.-B. Manneville, Jacques Prost, John Toner, M. D. Houslay, P. B. Canham, P. B. Sunil Kumar, P.-G. de Gennes, R. Lipowsky, S. Levin, S. Tuvia, Sriram Ramaswamy, U. Seifert, W. Cai, W. Helfrich, Y. Marathe   +23 more
core   +2 more sources

Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface.

Cell, 1992
We show that a protein with a glycosylphosphatidyl inositol (GPI) anchor can be recovered from lysates of epithelial cells in a low density, detergent-insoluble form.
Deborah A. Brown, J. Rose
semanticscholar   +1 more source

Directed Mutational Strategies Reveal Drug Binding and Transport by the MDR Transporters of Candida albicans

Journal of Fungi, 2021
Multidrug resistance (MDR) transporters belonging to either the ATP-Binding Cassette (ABC) or Major Facilitator Superfamily (MFS) groups are major determinants of clinical drug resistance in fungi.
Atanu Banerjee, Jorgaq Pata, Suman Sharma, Brian C. Monk, Pierre Falson, Rajendra Prasad   +5 more
doaj   +1 more source

Phase separation of integral membrane proteins in Triton X-114 solution.

Journal of Biological Chemistry, 1981
A solution of the nonionic detergent Triton X-114 is homogeneous at 0 degrees C but separates in an aqueous phase and a detergent phase above 20 degrees C.
C. Bordier
semanticscholar   +1 more source

Spontaneous Suppressors against Debilitating Transmembrane Mutants of CaMdr1 Disclose Novel Interdomain Communication via Signature Motifs of the Major Facilitator Superfamily

Journal of Fungi, 2022
The Major Facilitator Superfamily (MFS) drug:H+ antiporter CaMdr1, from Candida albicans, is responsible for the efflux of structurally diverse antifungals.
Suman Sharma, Atanu Banerjee, Alexis Moreno, Archana Kumari Redhu, Pierre Falson, Rajendra Prasad   +5 more
doaj   +1 more source

Membrane proteins bind lipids selectively to modulate their structure and function

Nature, 2014
Previous studies have established that the folding, structure and function of membrane proteins are influenced by their lipid environments and that lipids can bind to specific sites, for example, in potassium channels.
A. Laganowsky, Eamonn Reading, Timothy M. Allison, M. Ulmschneider, M. Degiacomi, A. Baldwin, C. Robinson   +6 more
semanticscholar   +1 more source

A morphological view on mitochondrial protein targeting [PDF]

, 1994
Mitochondrial protein targeting includes both intramitochondrial sorting of proteins encoded by the organellar genome and import and subsequent sorting of nuclear encoded precursor proteins. Only a few proteins are encoded by the mitochondrial genome and
Ades, Allmann, Ardail, Ardail, Ardail, Attardi, Baker, Biermans, Biermans, Brdiczka, Brdiczka, Eilers, Glick, Hackenbrock, Hackenbrock, Hackenbrock, Hartl, Hartl, Hines, Hwang, Kellems, Kiebler, Knoll, Lill, Lithgow, Malhotra, Martin, Marzuki, McEnery, Murakami, Nicolay, Ohlendieck, Pain, Pfanner, Pfanner, Pfanner, Pon, Rassow, Rassow, Rassow, Rojo, Schleyer, Schwaiger, Stuart, Suissa, Söllner, Söllner, Söllner, van Venetië, Vestweber, Vestweber, Vignais, Watson, Werner, Wienhues, Zara   +55 more
core   +1 more source