Results 11 to 20 of about 1,969,206 (269)

In vitro reconstitution of substrate S-acylation by the zDHHC family of protein acyltransferases

Open Biology, 2022
Protein S-acylation, more commonly known as protein palmitoylation, is a biological process defined by the covalent attachment of long chain fatty acids onto cysteine residues of a protein, effectively altering the local hydrophobicity and influencing ...
R. Elliot Murphy, Anirban Banerjee
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Structure and dynamics of semaglutide- and taspoglutide-bound GLP-1R-Gs complexes

Cell Reports, 2021
Summary: The glucagon-like peptide-1 receptor (GLP-1R) regulates insulin secretion, carbohydrate metabolism, and appetite and is an important target for treatment of type 2 diabetes and obesity. Multiple GLP-1R agonists have entered into clinical trials,
Xin Zhang, Matthew J. Belousoff, Yi-Lynn Liang, Radostin Danev, Patrick M. Sexton, Denise Wootten   +5 more
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Pharmacological hallmarks of allostery at the M4 muscarinic receptor elucidated through structure and dynamics

eLife, 2023
Allosteric modulation of G protein-coupled receptors (GPCRs) is a major paradigm in drug discovery. Despite decades of research, a molecular-level understanding of the general principles that govern the myriad pharmacological effects exerted by GPCR ...
Ziva Vuckovic, Jinan Wang, Vi Pham, Jesse I Mobbs, Matthew J Belousoff, Apurba Bhattarai, Wessel AC Burger, Geoff Thompson, Mahmuda Yeasmin, Vindhya Nawaratne, Katie Leach, Emma T van der Westhuizen, Elham Khajehali, Yi-Lynn Liang, Alisa Glukhova, Denise Wootten, Craig W Lindsley, Andrew Tobin, Patrick Sexton, Radostin Danev, Celine Valant, Yinglong Miao, Arthur Christopoulos, David M Thal   +23 more
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Fluorescently tagged nanobodies and NanoBRET to study ligand-binding and agonist-induced conformational changes of full-length EGFR expressed in living cells

Frontiers in Immunology, 2022
IntroductionThe Epidermal Growth Factor Receptor is a member of the Erb receptor tyrosine kinase family. It binds several ligands including EGF, betacellulin (BTC) and TGF-α, controls cellular proliferation and invasion and is overexpressed in various ...
Dehan Comez, Dehan Comez, Jacqueline Glenn, Jacqueline Glenn, Stephanie M. Anbuhl, Stephanie M. Anbuhl, Raimond Heukers, Raimond Heukers, Martine J. Smit, Stephen J. Hill, Stephen J. Hill, Laura E. Kilpatrick, Laura E. Kilpatrick   +12 more
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Informing β-cell regeneration strategies using studies of heterogeneity

Molecular Metabolism, 2019
Background: Current therapeutic strategies for type 1 (T1DM) and type 2 diabetes mellitus (T2DM) rely on increasing or substituting endogenous insulin secretion in combination with lifestyle changes.
Daniela Nasteska, Katrina Viloria, Lewis Everett, David J. Hodson   +3 more
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Structure of Human ATG9A, the Only Transmembrane Protein of the Core Autophagy Machinery

Cell Reports, 2020
Summary: Autophagy is a catabolic process involving capture of cytoplasmic materials into double-membraned autophagosomes that subsequently fuse with lysosomes for degradation of the materials by lysosomal hydrolases.
Carlos M. Guardia, Xiao-Feng Tan, Tengfei Lian, Mitra S. Rana, Wenchang Zhou, Eric T. Christenson, Augustus J. Lowry, José D. Faraldo-Gómez, Juan S. Bonifacino, Jiansen Jiang, Anirban Banerjee   +10 more
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Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics

Nature Communications, 2020
Glucagon-like peptide-1 receptor is an important regulator of appetite and glucose homeostasis. Here the authors describe super-resolution microscopy and in vivo imaging compatible fluorescent probes, which reveal endogenous glucagon-like peptide-1 ...
Julia Ast, Anastasia Arvaniti, Nicholas H. F. Fine, Daniela Nasteska, Fiona B. Ashford, Zania Stamataki, Zsombor Koszegi, Andrea Bacon, Ben J. Jones, Maria A. Lucey, Shugo Sasaki, Daniel I. Brierley, Benoit Hastoy, Alejandra Tomas, Giuseppe D’Agostino, Frank Reimann, Francis C. Lynn, Christopher A. Reissaus, Amelia K. Linnemann, Elisa D’Este, Davide Calebiro, Stefan Trapp, Kai Johnsson, Tom Podewin, Johannes Broichhagen, David J. Hodson   +25 more
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Author Correction: Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics

Nature Communications, 2020
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Julia Ast, Anastasia Arvaniti, Nicholas H. F. Fine, Daniela Nasteska, Fiona B. Ashford, Zania Stamataki, Zsombor Koszegi, Andrea Bacon, Ben J. Jones, Maria A. Lucey, Shugo Sasaki, Daniel I. Brierley, Benoit Hastoy, Alejandra Tomas, Giuseppe D’Agostino, Frank Reimann, Francis C. Lynn, Christopher A. Reissaus, Amelia K. Linnemann, Elisa D’Este, Davide Calebiro, Stefan Trapp, Kai Johnsson, Tom Podewin, Johannes Broichhagen, David J. Hodson   +25 more
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High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach

Bio-Protocol, 2020
Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is
Franziska Heydenreich, Tamara Miljuš, Dalibor Milić, Dmitry Veprintsev   +3 more
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Directed Mutational Strategies Reveal Drug Binding and Transport by the MDR Transporters of Candida albicans

Journal of Fungi, 2021
Multidrug resistance (MDR) transporters belonging to either the ATP-Binding Cassette (ABC) or Major Facilitator Superfamily (MFS) groups are major determinants of clinical drug resistance in fungi.
Atanu Banerjee, Jorgaq Pata, Suman Sharma, Brian C. Monk, Pierre Falson, Rajendra Prasad   +5 more
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