Results 21 to 30 of about 2,118,036 (319)

In vitro reconstitution of substrate S-acylation by the zDHHC family of protein acyltransferases

Open Biology, 2022
Protein S-acylation, more commonly known as protein palmitoylation, is a biological process defined by the covalent attachment of long chain fatty acids onto cysteine residues of a protein, effectively altering the local hydrophobicity and influencing ...
R. Elliot Murphy, Anirban Banerjee
doaj   +1 more source

Informing β-cell regeneration strategies using studies of heterogeneity

Molecular Metabolism, 2019
Background: Current therapeutic strategies for type 1 (T1DM) and type 2 diabetes mellitus (T2DM) rely on increasing or substituting endogenous insulin secretion in combination with lifestyle changes.
Daniela Nasteska, Katrina Viloria, Lewis Everett, David J. Hodson   +3 more
doaj   +1 more source

Fluorescently tagged nanobodies and NanoBRET to study ligand-binding and agonist-induced conformational changes of full-length EGFR expressed in living cells

Frontiers in Immunology, 2022
IntroductionThe Epidermal Growth Factor Receptor is a member of the Erb receptor tyrosine kinase family. It binds several ligands including EGF, betacellulin (BTC) and TGF-α, controls cellular proliferation and invasion and is overexpressed in various ...
Dehan Comez, Dehan Comez, Jacqueline Glenn, Jacqueline Glenn, Stephanie M. Anbuhl, Stephanie M. Anbuhl, Raimond Heukers, Raimond Heukers, Martine J. Smit, Stephen J. Hill, Stephen J. Hill, Laura E. Kilpatrick, Laura E. Kilpatrick   +12 more
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The yeast vacuolar membrane proteome [PDF]

, 2009
Transport of solutes between the cytosol and the vacuolar lumen is of crucial importance for various functions of vacuoles, including ion homeostasis; detoxification; storage of different molecules such as amino acids, phosphate, and calcium ions; and ...
Poolman, Bert,, Slotboom, Dirk J.,, Breitling, Rainer, Permentier, Hjalmar P.,, Gandhi, Tejas, Breitling, R., Breitling, Rainer,, Slotboom, D.J., Gandhi, Tejas,, Permentier, Hjalmar P., Permentier, H.P., Gandhi, T., Wiederhold, Elena,, Poolman, Bert, Wiederhold, Elena, Wiederhold, E., Poolman, B., Permentier, Hjalmar P.; id_orcid, Slotboom, Dirk J.   +18 more
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Structure of Human ATG9A, the Only Transmembrane Protein of the Core Autophagy Machinery

Cell Reports, 2020
Summary: Autophagy is a catabolic process involving capture of cytoplasmic materials into double-membraned autophagosomes that subsequently fuse with lysosomes for degradation of the materials by lysosomal hydrolases.
Carlos M. Guardia, Xiao-Feng Tan, Tengfei Lian, Mitra S. Rana, Wenchang Zhou, Eric T. Christenson, Augustus J. Lowry, José D. Faraldo-Gómez, Juan S. Bonifacino, Jiansen Jiang, Anirban Banerjee   +10 more
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The use of SMALPs as a novel membrane protein scaffold for structure study by negative stain electron microscopy. [PDF]

, 2015
Despite the great progress recently made in resolving their structures, investigation of the structural biology of membrane proteins still presents major challenges.
Shaun Rawson, Mitchell, JK, Postis, Vincent; id_orcid, Vincent Postis, Tim R Dafforn, Postis, Vincent, Muench, Stephen P., Baldwin, SA, Rawson, Shaun, Baldwin, Stephen A., Postis, V, Rawson, S, Stephen A Baldwin, Lee, SC, Rosemary A Parslow, Mitchell, Jennifer K., Dafforn, TR, Lee, Sarah C., Parslow, Rosemary A., Jennifer K Mitchell, Sarah C Lee, Stephen P Muench, Muench, SP, Dafforn, Tim R., Parslow, RA   +24 more
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Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics

Nature Communications, 2020
Glucagon-like peptide-1 receptor is an important regulator of appetite and glucose homeostasis. Here the authors describe super-resolution microscopy and in vivo imaging compatible fluorescent probes, which reveal endogenous glucagon-like peptide-1 ...
Julia Ast, Anastasia Arvaniti, Nicholas H. F. Fine, Daniela Nasteska, Fiona B. Ashford, Zania Stamataki, Zsombor Koszegi, Andrea Bacon, Ben J. Jones, Maria A. Lucey, Shugo Sasaki, Daniel I. Brierley, Benoit Hastoy, Alejandra Tomas, Giuseppe D’Agostino, Frank Reimann, Francis C. Lynn, Christopher A. Reissaus, Amelia K. Linnemann, Elisa D’Este, Davide Calebiro, Stefan Trapp, Kai Johnsson, Tom Podewin, Johannes Broichhagen, David J. Hodson   +25 more
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Author Correction: Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics

Nature Communications, 2020
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Julia Ast, Anastasia Arvaniti, Nicholas H. F. Fine, Daniela Nasteska, Fiona B. Ashford, Zania Stamataki, Zsombor Koszegi, Andrea Bacon, Ben J. Jones, Maria A. Lucey, Shugo Sasaki, Daniel I. Brierley, Benoit Hastoy, Alejandra Tomas, Giuseppe D’Agostino, Frank Reimann, Francis C. Lynn, Christopher A. Reissaus, Amelia K. Linnemann, Elisa D’Este, Davide Calebiro, Stefan Trapp, Kai Johnsson, Tom Podewin, Johannes Broichhagen, David J. Hodson   +25 more
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Translocation and insertion of precursor proteins into isolated outer membranes of mitochondria [PDF]

, 1993
Nuclear-encoded proteins destined for mitochondria must cross the outer or both outer and inner membranes to reach their final sub- mitochondrial locations. While the inner membrane can translocate preproteins by itself, it is not known whether the outer
W Neupert, Lill, Roland, Neupert, Walter, A Mayer, Mayer, Andreas, R Lill   +5 more
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High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach

Bio-Protocol, 2020
Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is
Franziska Heydenreich, Tamara Miljuš, Dalibor Milić, Dmitry Veprintsev   +3 more
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