Results 91 to 100 of about 1,047,399 (305)

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Compartmentalization and Transport in Synthetic Vesicles

Frontiers in Bioengineering and Biotechnology, 2016
Nano-scale vesicles have become a popular tool in life sciences. Besides liposomes that are generated from phospholipids of natural origin, polymersomes fabricated of synthetic block copolymers enjoy increasing popularity, as they represent more ...
Christine eSchmitt, Anna H. Lippert, Navid eBonakdar, Vahid eSandoghdar, Lars Matthias Voll   +4 more
doaj   +1 more source

Solute channels of the outer membrane: from bacteria to chloroplasts [PDF]

, 2007
Chloroplasts, unique organelles of plants, originated from endosymbiosis of an ancestor of today's cyanobacteria with a mitochondria-containing host cell. It is assumed that the outer envelope membrane, which delimits the chloroplast from the surrounding
Aljamal J.A., Benz R., Blumenthal A., Drea S.C., Fischer K., Murcha M.W., Pilsl H., Umeda H., Zizi M.   +8 more
core   +1 more source

pH‐mediated activation of the lysosomal arginine sensor SLC38A9

FEBS Letters, EarlyView.
Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley   +1 more source

"Alternative" endocytic mechanisms exploited by pathogens: new avenues for therapeutic delivery? [PDF]

, 2007
Some pathogens utilize unique routes to enter cells that may evade the intracellular barriers encountered by the typical clathrin-mediated endocytic pathway.
Medina-Kauwe, LK
core   +1 more source

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

IDRs in Cross-Membrane Transport: Regulation of Ion Channels and Transporters, Mechanistic Studies Made Possible by NMR and Computational Methods

Frontiers in Biophysics
The roles of intrinsically disordered proteins (IDPs) and regions (IDRs) in health and disease have gained increasing attention in recent years. Understanding their structure–activity relationships remains challenging, especially for integral membrane ...
Olga Vinogradova
doaj   +1 more source

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

Molecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal   +16 more
wiley   +1 more source

An Optimized Screen Reduces the Number of GA Transporters and Provides Insights Into Nitrate Transporter 1/Peptide Transporter Family Substrate Determinants

Frontiers in Plant Science, 2019
Based on recent in vitro data, a relatively large number of the plant nitrate transporter 1/peptide transporter family (NPF) proteins have been suggested to function as gibberellic acid (GA) transporters.
Nikolai Wulff, Heidi Asschenfeldt Ernst, Morten Egevang Jørgensen, Morten Egevang Jørgensen, Morten Egevang Jørgensen, Sophie Lambertz, Tobias Maierhofer, Zeinu Mussa Belew, Christoph Crocoll, Mohammed Saddik Motawia, Dietmar Geiger, Flemming Steen Jørgensen, Osman Mirza, Hussam Hassan Nour-Eldin   +13 more
doaj   +1 more source

Unidirectional Reconstitution into Detergent-destabilized Liposomes of the Purified Lactose Transport System of Streptococcus thermophilus [PDF]

, 1996
The lactose transport protein (LacS) of Streptococcus thermophilus was amplified to levels as high as 8 and 30% of total membrane protein in Escherichia coli and S. thermophilus, respectively.
Henderson, Peter J.F.,, Knol, Jan,, Leblanc, Gérard,, Liang, Wei-Jun,, Poolman, Bert,, Veenhoff, Liesbeth,   +5 more
core   +2 more sources