Results 151 to 160 of about 224,221 (263)

Mild C─F Activation of Azido(per)Fluoroalkanes

Chemistry – A European Journal, EarlyView.
Mild and selective activation of C(sp3)–F bonds in azido(per)fluoroalkanes using thiolates was developed, leading to novel functionalized azides. The reaction is initiated by nucleophilic attack of the sulfur atom to the terminal nitrogen of the azido group, followed by fluoride ion elimination.
Olena Shaitanova, María Martínez Mallo, Sergii Suikov, Blanka Klepetářová, Josef Filgas, Petr Slavíček, Lucie Bednárová, Petr Beier   +7 more
wiley   +1 more source

Protocol for minimizing necrotic core formation in iPSC-derived cortical organoids. [PDF]

STAR Protoc
Mamun MMA, Chetty S.
europepmc   +1 more source

Fibrilpaint: A Class of Amyloid‐Targeting Peptides

Chemistry – A European Journal, EarlyView.
FibrilPaints are modular peptides engineered to recognize amyloid fibrils. By systematically varying charge, sequence, and termini, their binding behavior can be tuned while retaining recognition. Successful binding ″paints″ fibrils for visualization and opens routes toward targeted degradation and diagnostic applications.
Júlia Aragonès Pedrola, Françoise A. Dekker, Katrin Guttmann, Litske M. van Leeuwen, Shalini Singh, Guy Mayer, Tommaso Garfagnini, Assaf Friedler, Stefan G. D. Rüdiger   +8 more
wiley   +1 more source

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source

Protocol for synthesizing and purifying short-length poly(ADP)ribose polymer using fast protein liquid chromatography. [PDF]

STAR Protoc
Neeharika S, Hanuman DS, Rajakumara E.
europepmc   +1 more source

Isoform Specificity of a Compound Targeting Actin Filaments Containing Tropomyosin Tpm1.8/1.9

Cytoskeleton, EarlyView.
ABSTRACT The unbranched actin filaments in mammalian cells are usually composed of co‐polymers of a specific tropomyosin isoform with actin. Genetic manipulation has revealed that the tropomyosins largely define the functional properties of actin filaments in an isoform‐specific, non‐redundant manner.
Jeff Hook, Edna C. Hardeman, Peter W. Gunning   +2 more
wiley   +1 more source

The Influence of Drosophila Spire and Myosin V During Mid‐Oogenesis Is Independent of Their Direct Interaction

Cytoskeleton, EarlyView.
ABSTRACT Cooperativity between cytoskeletal proteins is crucial for spatiotemporal coordination in biological processes, like oogenesis. In mammalian and Drosophila oogenesis, proper assembly and function of actin networks require coordination between actin assembly factors Spire and formins, as well as actin‐associated proteins like myosins and Rab ...
Joseph Y. Ong, Hannah M. Bailey, Emma Carley, Michelle Panzica, Merin Rixen, Neil Billington, Rong Liu, Margot E. Quinlan   +7 more
wiley   +1 more source

Ascorbic acid enhances <i>in vitro</i> primordial germ cell-like cell differentiation from mouse ESCs. [PDF]

Front Cell Dev Biol
Kumar U, Kumar N, Chandra Shekar P, Thangaraj K.   +3 more
europepmc   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

Expression and purification of Steroidogenic Factor-1 (NR5A1) nuclear receptor ligand binding domain complexed with the signaling phosphoinositide lipid PI(3,4,5)P₃. [PDF]

Methods Enzymol
Campbell AN, Cutright AJ, Martin J, Kheda A, Loi MNC, Blind RD.   +5 more
europepmc   +1 more source