A cost‐effective DNA extraction protocol for long‐read sequencing in non‐model plants
Abstract Premise Third‐generation sequencing has revolutionized genomics, enabling in‐depth analysis of genome sequence, structure, and epigenetic features. Yet, extracting high‐quality DNA for long‐read sequencing remains a bottleneck—particularly in non‐model plants, such as mature trees growing in natural environments, which often contain abundant ...
Sofia Gaischuk +6 more
wiley +1 more source
Transformations of perfluorotoluene by the action of 2-mercaptoethanol
Borislav V. Koshcheev +4 more
doaj +1 more source
Biochemical characterisation of a new thermotolerant L-asparaginase produced by Cunninghamella echinulata PA3S12MM isolated from the Atlantic Forest. [PDF]
Von Groll S +9 more
europepmc +1 more source
Objective Immune complexes (ICs), formed by autoantigen and autoantibody, play a pathogenic role in systemic autoimmune diseases through stimulation of Fcγ receptors (FcγR). However, studies investigating bioactivity of circulating ICs across various diseases remain limited.
Koji Suzuki +8 more
wiley +1 more source
Tandem RNA and Protein Extraction: A Platform for Maximizing the Use of Limited Ex Vivo Tissue Samples. [PDF]
Kennedy C +9 more
europepmc +1 more source
Objective Microbial small RNAs can regulate human genes. Higher plasma concentrations of microbial transfer RNA (tRNA)‐derived RNA‐1 (tDR‐1) were previously associated with lower rheumatoid arthritis (RA) disease activity. This study examined whether tDR‐1 concentrations differ in anticyclic citrullinated peptide‐3 positive (CCP3+) at‐risk individuals (
Anastasiia Phothisane +11 more
wiley +1 more source
Screening and Targeting the HMGB1/RAGE Axis via Proteomics to Alleviate Inflammatory Responses in a <i>Pseudomonas aeruginosa</i>-Induced Rat Model of VAP. [PDF]
Huang X +7 more
europepmc +1 more source
Harnessing plant‐based platform for low‐cost cellulosic sugar recovery from bioenergy crops
Abstract The price of pure cellulase enzyme for the recovery of fermentable cellulosic sugars is one of the major challenges that limit the commercialization of second‐generation biofuels and bio‐based products. This work shows a means to greatly reduce the cost of cellulases. The abundant capacity of plants to synthesize and hyperaccumulate transgenic
Shraddha Maitra +4 more
wiley +1 more source
Computational Repurposing and Experimental Validation of YBX1 Inhibitors in Hepatocellular Carcinoma. [PDF]
Karkoutly O +3 more
europepmc +1 more source
Potential Alteration of COVID-19 by Beta-Mercaptoethanol
openaire +3 more sources

