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Metagenome-assembled genomes: concepts, analogies, and challenges
Biophysical Reviews, 2021Metagenome-assembled genomes (MAGs) are microbial genomes reconstructed from metagenome data. In the last few years, many thousands of MAGs have been reported in the literature, for a variety of environments and host-associated microbiota, including humans.
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Recovery and Analysis of Long-Read Metagenome-Assembled Genomes
2023The development of long-read nucleic acid sequencing is beginning to make very substantive impact on the conduct of metagenome analysis, particularly in relation to the problem of recovering the genomes of member species of complex microbial communities.
Krithika, Arumugam +3 more
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266 cultured genomes and 482 metagenome-assembled genomes
2021We performed a study of bacteria cultivation and genome sequencing along with deeply metagenome sequencing of 14 fecal samples from weanling piglet. 266 cultured genomes and 482 metagenome-assembled genomes (MAGs) were collected and clustered to 428 species across 10 phylum in this work. Genomic DNA of the 226 represent strains were extracted.
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ACR: metagenome-assembled prokaryotic and eukaryotic genome refinement tool
Briefings in Bioinformatics, 2023Abstract Microbial genome recovery from metagenomes can further explain microbial ecosystem structures, functions and dynamics. Thus, this study developed the Additional Clustering Refiner (ACR) to enhance high-purity prokaryotic and eukaryotic metagenome-assembled genome (MAGs) recovery.
Hoon Je Seong, Jin Ju Kim, Woo Jun Sul
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CRISPR detection in metagenome-assembled genomes (MAGs) of coal mine
Functional & Integrative Genomics, 2023Bacterial and archaeal CRISPR-Cas systems provide adaptive immune protection against foreign mobile genetic elements. When viruses infect bacteria, a small portion of the viral DNA is inserted into the bacterial DNA in a specific pattern to produce segments known as CRISPR arrays.
Arunmozhi Bharathi, Achudhan +2 more
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How to Obtain and Compare Metagenome-Assembled Genomes
Metagenome-assembled genomes, or MAGs, are genomes retrieved from metagenome datasets. In the vast majority of cases, MAGs are genomes from prokaryotic species that have not been isolated or cultivated in the lab. They, therefore, provide us with information on these species that are impossible to obtain otherwise, at least until new cultivation ...Fabio Beltrame Sanchez +2 more
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CheckV Assesses the Quality and Completeness of Metagenome-Assembled Viral Genomes
Nature Biotechnology, 2021Frederik Schulz +2 more
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